GLS sirna

GLS siRNA (Human)

Cat. Num. AAA8206049

• Gene Names: GLS; GAC; GAM; KGA; GLS1; AAD20
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GLS; GLS siRNA (Human); GLS1; KIAA0838; Glutaminase kidney isoform mitochondrial; K-glutaminase; L-glutamine amidohydrolase; GLS sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GLS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 598

• Applicable Applications for GLS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GLS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GLS sirna

siRNA to inhibit GLS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 373251164
• NCBI GeneID: 2744
• NCBI Accession #: NP_001243239.1
• NCBI GenBank Nucleotide #: NM_001256310.1
• UniProt Accession #: O94925; Q9UL05; Q9UL06; Q9UL07; Q9UN40
• Molecular Weight: 65,460 Da
• NCBI Official Full Name: glutaminase kidney isoform, mitochondrial isoform 2
• NCBI Official Synonym Full Names: glutaminase
• NCBI Official Symbol: GLS
• NCBI Official Synonym Symbols: GAC; GAM; KGA; GLS1; AAD20
• NCBI Protein Information: glutaminase kidney isoform, mitochondrial
• UniProt Protein Name: Glutaminase kidney isoform, mitochondrial
• Protein Family: Glutaminase kidney
• UniProt Gene Name: GLS
• UniProt Synonym Gene Names: GLS1; KIAA0838; GLS
• UniProt Entry Name: GLSK_HUMAN

NCBI Description

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

glutaminase: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Belongs to the glutaminase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Energy Metabolism - nitrogen; Hydrolase; Mitochondrial; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; EC 3.5.1.2

Chromosomal Location of Human Ortholog: 2q32-q34

Cellular Component: mitochondrion; mitochondrial matrix; cytosol

Molecular Function: protein binding; glutaminase activity

Biological Process: synaptic transmission; glutamate secretion; suckling behavior; neurotransmitter secretion; glutamine catabolic process; neurological control of breathing; glutamate biosynthetic process; amino acid biosynthetic process; protein homotetramerization