Human glutaminase ELISA Kit | GLS elisa kit

Human Glutaminase kidney isoform, mitochondrial, GLS ELISA Kit

Cat. Num. AAA2602981

• Gene Names: GLS; GAC; GAM; KGA; GLS1; AAD20
• Reactivity: Human
• Synonyms: glutaminase; Human Glutaminase kidney isoform; mitochondrial; GLS ELISA Kit; GLS elisa kit

• Reactivity: Human

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GLS elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human GLS monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 373251164
• NCBI GeneID: 2744
• NCBI Accession #: NP_001243239.1
• NCBI GenBank Nucleotide #: NM_001256310.1
• UniProt Accession #: O94925; Q9UL05; Q9UL06; Q9UL07; Q9UN40
• Molecular Weight: 65,460 Da
• NCBI Official Full Name: glutaminase kidney isoform, mitochondrial isoform 2
• NCBI Official Synonym Full Names: glutaminase
• NCBI Official Symbol: GLS
• NCBI Official Synonym Symbols: GAC; GAM; KGA; GLS1; AAD20
• NCBI Protein Information: glutaminase kidney isoform, mitochondrial; K-glutaminase; glutaminase C; L-glutamine amidohydrolase; glutaminase, phosphate-activated
• UniProt Protein Name: Glutaminase kidney isoform, mitochondrial
• Protein Family: Glutaminase
• UniProt Gene Name: GLS
• UniProt Synonym Gene Names: GLS1; KIAA0838; GLS
• UniProt Entry Name: GLSK_HUMAN

NCBI Description

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

glutaminase: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Belongs to the glutaminase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 3.5.1.2; Hydrolase; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 2q32-q34

Cellular Component: mitochondrion; mitochondrial matrix; cytosol

Molecular Function: protein binding; glutaminase activity

Biological Process: synaptic transmission; glutamate secretion; suckling behavior; neurotransmitter secretion; glutamine catabolic process; neurological control of breathing; glutamate biosynthetic process; amino acid biosynthetic process; protein homotetramerization