Mouse Insulin Receptor (INSR) ELISA Kit | INSR elisa kit

Mouse Insulin Receptor (INSR) Mini Samples ELISA Kit

Cat. Num. AAA2708334

• Gene Names: Insr; IR; IR-A; IR-B; CD220; 4932439J01Rik; D630014A15Rik
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Insulin Receptor (INSR); Mouse Insulin Receptor (INSR) Mini Samples ELISA Kit; Insulin Receptor; CD220; ISR; HHF5; IR; INSR elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Insulin Receptor (INSR).; No significant cross-reactivity or interference between Mini Samples Insulin Receptor (INSR) and analogues was observed.
• Sequence Length: 1372

• Applicable Applications for INSR elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.117ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Insulin Receptor (INSR) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Insulin Receptor (INSR) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Insulin Receptor (INSR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Insulin Receptor (INSR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Insulin Receptor (INSR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Insulin Receptor (INSR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 157057178
• NCBI GeneID: 16337
• NCBI Accession #: NP_034698
• NCBI GenBank Nucleotide #: NP_034698.2
• UniProt Accession #: P15208
• NCBI Official Full Name: insulin receptor isoform A preproprotein
• NCBI Official Synonym Full Names: insulin receptor
• NCBI Official Symbol: Insr
• NCBI Official Synonym Symbols: IR; IR-A; IR-B; CD220; 4932439J01Rik; D630014A15Rik
• NCBI Protein Information: insulin receptor
• UniProt Protein Name: Insulin receptor
• Protein Family: Insulin receptor
• UniProt Gene Name: Insr
• UniProt Synonym Gene Names: IR
• UniProt Entry Name: INSR_MOUSE

NCBI Description

This gene encodes a member of the receptor tyrosine kinase family of transmembrane signaling proteins that play important roles in cell differentiation, growth and metabolism. The encoded preproprotein undergoes proteolytic processing to generate alpha and beta chains that form a disulfide-linked heterodimer which, in turn homodimerizes to form a mature, functional receptor. Mice lacking the encoded protein develop severe hyperglycemia and hyperketonemia, and die within a couple of days after birth as a result of diabetic ketoacidosis. [provided by RefSeq, Aug 2016]

Uniprot Description

INSR: a receptor tyrosine kinase that mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). The holoenzyme is cleaved into two chains, the alpha and beta subunits. The active complex is a tetramer containing 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains constitute the ligand- binding domain, while the beta chains carry the kinase domain. Interacts with SORBS1 but dissociates from it following insulin stimulation. Familial mutations associated with insulin resistant diabetes, acanthosis nigricans, pineal hyperplasia, and polycystic ovary syndrome. SNP variants may be associated with polycystic ovary syndrome, atypical migraine and diabetic hyperlipidemia. Mutations also cause leprechaunism, a severe insulin resistance syndrome causing growth retardation and death in early infancy. Two isoforms of the human protein are produced by alternative splicing. The Short isoform has a higher affinity for insulin than the longer. Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta. Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas.

Protein type: EC 2.7.10.1; Protein kinase, TK; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; InsR family

Cellular Component: membrane; intracellular membrane-bound organelle; integral to plasma membrane; integral to membrane; plasma membrane; synapse; caveola; nucleus; cytosol; endosome; receptor complex

Molecular Function: protein domain specific binding; GTP binding; PTB domain binding; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; insulin-like growth factor II binding; protein kinase binding; protein phosphatase binding; lipoic acid binding; protein kinase activity; transferase activity; insulin binding; insulin-like growth factor receptor binding; protein binding; insulin-like growth factor I binding; insulin receptor substrate binding; 3-phosphoinositide-dependent protein kinase binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; protein complex binding; phosphoinositide 3-kinase binding; kinase activity; ATP binding; insulin receptor activity

Biological Process: heart morphogenesis; epidermis development; positive regulation of nitric oxide biosynthetic process; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; positive regulation of glycogen biosynthetic process; regulation of embryonic development; exocrine pancreas development; glucose homeostasis; protein amino acid phosphorylation; positive regulation of glucose import; positive regulation of MAPKKK cascade; negative regulation of protein amino acid phosphorylation; regulation of transcription, DNA-dependent; male sex determination; positive regulation of cell proliferation; protein heterotetramerization; regulation of hydrogen peroxide metabolic process; positive regulation of developmental growth; positive regulation of mitosis; activation of protein kinase B; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; organ morphogenesis; cellular response to insulin stimulus; positive regulation of glycolysis; activation of protein kinase activity; insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation; phosphorylation; negative regulation of transporter activity; positive regulation of DNA replication; positive regulation of phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; transformation of host cell by virus; positive regulation of cell migration