FZD7 sirna

FZD7 siRNA (Human)

Cat. Num. AAA8236824

• Gene Names: FZD7; FzE3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FZD7; FZD7 siRNA (Human); Frizzled-7; Fz-7; hFz7; FzE3; FZD7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FZD7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 574

• Applicable Applications for FZD7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FZD7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FZD7 sirna

siRNA to inhibit FZD7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4503833
• NCBI GeneID: 8324
• NCBI Accession #: NP_003498.1
• NCBI GenBank Nucleotide #: NM_003507.1
• UniProt Accession #: O75084; O94816; Q53S59; Q96B74
• Molecular Weight: 63,620 Da
• NCBI Official Full Name: frizzled-7
• NCBI Official Synonym Full Names: frizzled class receptor 7
• NCBI Official Symbol: FZD7
• NCBI Official Synonym Symbols: FzE3
• NCBI Protein Information: frizzled-7
• UniProt Protein Name: Frizzled-7
• Protein Family: Frizzled
• UniProt Gene Name: FZD7
• UniProt Synonym Gene Names: Fz-7; hFz7
• UniProt Entry Name: FZD7_HUMAN

NCBI Description

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD7: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: GPCR, Fz/Smo family; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; protein binding; Wnt receptor activity; frizzled binding; PDZ domain binding

Biological Process: neuron differentiation; G-protein coupled receptor protein signaling pathway; regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of transcription, DNA-dependent; stem cell maintenance; T cell differentiation in the thymus; somatic stem cell division; positive regulation of JNK cascade; Wnt receptor signaling pathway through beta-catenin; satellite cell compartment self-renewal involved in skeletal muscle regeneration; negative regulation of ectodermal cell fate specification; positive regulation of phosphorylation