Human Fc fragment of IgG, low affinity IIIb, receptor (CD16b) ELISA Kit | FCGR3B elisa kit

Human Low affinity immunoglobulin gamma Fc region receptor III-B, FCGR3B ELISA Kit

Cat. Num. AAA935927

• Gene Names: FCGR3B; CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
• Reactivity: Human
• Synonyms: Fc fragment of IgG; low affinity IIIb; receptor (CD16b); Human Low affinity immunoglobulin gamma Fc region receptor III-B; FCGR3B ELISA Kit; Human Low affinity immunoglobulin gamma Fc region receptor III-B (FCGR3B) ELISA kit; CD16; CD16b; FCG3; FCGR3; receptor for (CD16) ; Fc-gamma receptor IIIb (CD 16) ; low affinity immunoglobulin gamma Fc region receptor III-B; FCGR3B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human FCGR3B. No significant cross-reactivity or interference between human FCGR3B and analogues was observed.
• Sequence Length: 233

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 1.56 ng/ml-100 ng/ml
• Sensitivity: < 0.39 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FCGR3B elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FCGR3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FCGR3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FCGR3B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FCGR3B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 132814489
• NCBI GeneID: 2215
• NCBI Accession #: NP_000561.3
• NCBI GenBank Nucleotide #: NM_000570.4
• UniProt Accession #: O75015
• Molecular Weight: 26,216 Da
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor III-B isoform 2
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
• NCBI Official Symbol: FCGR3B
• NCBI Official Synonym Symbols: CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III-B; fc-gamma RIIIb; fc-gamma RIII-beta; igG Fc receptor III-1; Fc-gamma receptor IIIb (CD 16)
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-B
• Protein Family: Low affinity immunoglobulin gamma Fc region receptor
• UniProt Gene Name: FCGR3B
• UniProt Synonym Gene Names: CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
• UniProt Entry Name: FCG3B_HUMAN

NCBI Description

The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]

Uniprot Description

Function: Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.