Human SEPTIN7 (septin-7) ELISA Kit | SEPTIN7 elisa kit

Human SEPTIN7 (septin-7) ELISA Kit

Cat. Num. AAA7608179

• Gene Names: SEPT7; CDC3; CDC10; SEPT7A; NBLA02942
• Reactivity: Human
• Synonyms: SEPTIN7 (septin-7); Human SEPTIN7 (septin-7) ELISA Kit; CDC10; CDC10 protein homolog; CDC3; Nbla02942; SEPT7; SEPT7A; septin 7; SEPTIN7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SEPTIN7. No significant cross-reactivity or interference between SEPTIN7 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEPTIN7 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 148352331
• NCBI GeneID: 989
• NCBI Accession #: NP_001779.3
• NCBI GenBank Nucleotide #: NM_001788.5
• UniProt Accession #: Q16181; Q52M76; Q6NX50
• Molecular Weight: 50,680 Da
• NCBI Official Full Name: septin-7 isoform 1
• NCBI Official Synonym Full Names: septin 7
• NCBI Official Symbol: SEPT7
• NCBI Official Synonym Symbols: CDC3; CDC10; SEPT7A; NBLA02942
• NCBI Protein Information: septin-7; CDC10 protein homolog; CDC10 (cell division cycle 10, S. cerevisiae, homolog)
• UniProt Protein Name: Septin-7
• UniProt Gene Name: SEPT7
• UniProt Synonym Gene Names: CDC10
• UniProt Entry Name: SEPT7_HUMAN

NCBI Description

This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. [provided by RefSeq, Jul 2011]

Uniprot Description

SEPT7: Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Belongs to the septin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Hydrolase; Cytoskeletal

Chromosomal Location of Human Ortholog: 7p14.2

Cellular Component: cell cortex; actin cytoskeleton; microtubule cytoskeleton; cytoplasm; apical plasma membrane; plasma membrane; nucleolus; stress fiber; spindle; synapse; midbody; axoneme; nerve terminal; nucleus; cleavage furrow

Molecular Function: identical protein binding; protein binding; GTP binding; structural molecule activity

Biological Process: mitosis; protein heterooligomerization; cytokinesis; shape changes of embryonic cells