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Western Blot (WB) (Western blot analysis of extracts from Jukat cells, using Parkin antibody.)

Rabbit Parkin Antibody | anti-PRKN antibody

Parkin Antibody

Gene Names
PRKN; PDJ; AR-JP; LPRS2; PARK2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Parkin; Parkin Antibody; PARK2; PRKN2; Parkinson disease protein 2; Parkinson juvenile disease protein 2; ubiquitin E3 ligase PRKN; anti-PRKN antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
Specificity
Parkin antibody detects endogenous levels of total Parkin protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
465
Applicable Applications for anti-PRKN antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized peptide derived from human Parkin antibody.
Preparation and Storage
Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts from Jukat cells, using Parkin antibody.)

Western Blot (WB) (Western blot analysis of extracts from Jukat cells, using Parkin antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human brain tissue using Parkin antibody.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human brain tissue using Parkin antibody.)
References
Fang Yang, J. Biol. Chem., Apr 2005; 280: 17154-17162. Yukiko Kuroda, Hum. Mol. Genet., Mar 2006; 15: 883-895. Iris H. Henn, J. Neurosci., Feb 2007; 27: 1868-1878. Monica Joch, Mol. Biol. Cell, Jun 2007; 10.1091/mbc.E05-11-1027.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
E3 ubiquitin-protein ligase parkin isoform 1
NCBI Official Synonym Full Names
parkin RBR E3 ubiquitin protein ligase
NCBI Official Symbol
PRKN
NCBI Official Synonym Symbols
PDJ; AR-JP; LPRS2; PARK2
NCBI Protein Information
E3 ubiquitin-protein ligase parkin
UniProt Protein Name
E3 ubiquitin-protein ligase parkin
UniProt Gene Name
PRKN
UniProt Synonym Gene Names
Parkinson disease protein 2

NCBI Description

The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq, Jul 2008]

Uniprot Description

Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPT5, TOMM20, USP30, ZNF746 and AIMP2 (PubMed:10973942, PubMed:10888878, PubMed:11431533, PubMed:12150907, PubMed:12628165, PubMed:16135753, PubMed:21376232, PubMed:23754282, PubMed:23620051, PubMed:24660806, PubMed:24751536). Mediates monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context (PubMed:19229105, PubMed:20889974, PubMed:25621951). Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation (PubMed:17846173, PubMed:19229105). Mediates 'Lys-63'-linked polyubiquitination of a 22 kDa O-linked glycosylated isoform of SNCAIP, possibly playing a role in Lewy-body formation (PubMed:11590439, PubMed:11431533, PubMed:19229105, PubMed:11590439, PubMed:15728840). Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy (PubMed:20889974). Promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy) by promoting the ubiquitination of mitochondrial proteins such as TOMM20, RHOT1/MIRO1 and USP30 (PubMed:19029340, PubMed:19966284, PubMed:23620051, PubMed:24896179, PubMed:25527291). Preferentially assembles 'Lys-6'-, 'Lys-11'- and 'Lys-63'-linked polyubiquitin chains following mitochondrial damage, leading to mitophagy (PubMed:25621951). Mediates 'Lys-48'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in the regulation of neuron death (PubMed:21376232). Limits the production of reactive oxygen species (ROS). Regulates cyclin-E during neuronal apoptosis. In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress (PubMed:22082830). Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53 (PubMed:19801972). May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity (PubMed:11439185). May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene.

Research Articles on PRKN

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Product Notes

The PRKN prkn (Catalog #AAA856030) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Parkin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Parkin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000. Researchers should empirically determine the suitability of the PRKN prkn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Parkin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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