EPGN sirna

EPGN siRNA (Human)

Cat. Num. AAA828972

• Gene Names: EPGN; EPG; PRO9904; ALGV3072
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EPGN; EPGN siRNA (Human); Epigen; Epithelial mitogen; EPG; EPGN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EPGN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 154

• Applicable Applications for EPGN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EPGN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EPGN sirna

siRNA to inhibit EPGN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 401782583
• NCBI GeneID: 255324
• NCBI Accession #: NP_001257918.1
• NCBI GenBank Nucleotide #: NM_001270989.1
• UniProt Accession #: Q6UW88; A1BMM3; A1BMM4; A1BMM5; A1BMM6; A1BMM7; A1BMM8; A8K090
• Molecular Weight: 8,149 Da
• NCBI Official Full Name: epigen isoform 1
• NCBI Official Synonym Full Names: epithelial mitogen
• NCBI Official Symbol: EPGN
• NCBI Official Synonym Symbols: EPG; PRO9904; ALGV3072
• NCBI Protein Information: epigen
• UniProt Protein Name: Epigen
• Protein Family: Epigen
• UniProt Gene Name: EPGN
• UniProt Synonym Gene Names: EPG
• UniProt Entry Name: EPGN_HUMAN

NCBI Description

The protein encoded by this gene is a member of the epidermal growth factor family. Members of this family are ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration. This protein has been reported to have high mitogenic activity but low affinity for its receptor. Expression of this transcript and protein have been reported in cancer specimens of the breast, bladder, and prostate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

Epigen: Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; integral to plasma membrane

Molecular Function: growth factor activity; epidermal growth factor receptor binding

Biological Process: positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; activation of MAPK activity; positive regulation of cell proliferation; MAPKKK cascade; angiogenesis; positive regulation of epithelial cell proliferation