Human Aldo-keto Reductase Family 1 Member C2 (AKR1C2) ELISA Kit | AKR1C2 elisa kit

Human Aldo-keto Reductase Family 1 Member C2 (AKR1C2) ELISA Kit

Cat. Num. AAA9714305

• Gene Names: AKR1C2; DD; DD2; TDD; BABP; DD-2; DDH2; HBAB; HAKRD; MCDR2; SRXY8; DD/BABP; AKR1C-pseudo
• Reactivity: Human
• Synonyms: Aldo-keto Reductase Family 1 Member C2 (AKR1C2); Human Aldo-keto Reductase Family 1 Member C2 (AKR1C2) ELISA Kit; AKR1C2; AKR1C-pseudo; BABP; DD; DD2; DDH2; FLJ53800; HAKRD; HBAB; MCDR2; aldo-keto reductase family 1; member C2; chlordecone reductase homolog; pseudo-chlordecone reductase; trans-1; 2-dihydrobenzene-1; 2-diol; AKR1C2 elisa kit

• Reactivity: Human
• Specificity: Human Aldo-keto reductase family 1 member C2 (AKR1C2) ELISA Kit has high sensitivity and excellent specificity for detection of Human AKR1C2. No significant cross-reactivity or interference between Human AKR1C2 and analogues was observed.
• Sequence Length: 139

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Aldo-keto reductase family 1 member C2 (AKR1C2) Standard Curve.)

Related Product Information for AKR1C2 elisa kit

Description: This Human Aldo-keto reductase family 1 member C2 (AKR1C2) ELISA Kit employs a two-site sandwich ELISA to quantitate AKR1C2 in samples. An antibody specific for AKR1C2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AKR1C2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AKR1C2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKR1C2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Dihydrodiol dehydrogenase (DD; EC 1.3.1.20), a member of the aldo-oxo reductase (AKR) superfamily, catalyzes the NADP-linked oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols. DDH2 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

NCBI and Uniprot Product Information

• NCBI GI #: 207028673
• NCBI GeneID: 1646
• NCBI Accession #: NP_001128713.1
• NCBI GenBank Nucleotide #: NP_001128713.1
• UniProt Accession #: P52895
• NCBI Official Full Name: aldo-keto reductase family 1 member C2 isoform 2
• NCBI Official Synonym Full Names: aldo-keto reductase family 1 member C2
• NCBI Official Symbol: AKR1C2
• NCBI Official Synonym Symbols: DD; DD2; TDD; BABP; DD-2; DDH2; HBAB; HAKRD; MCDR2; SRXY8; DD/BABP; AKR1C-pseudo
• NCBI Protein Information: aldo-keto reductase family 1 member C2
• UniProt Protein Name: Aldo-keto reductase family 1 member C2
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: AKR1C2
• UniProt Synonym Gene Names: DDH2; DD-2; DD2; DD/BABP
• UniProt Entry Name: AK1C2_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

AKR1C2: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. Defects in AKR1C2 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. Belongs to the aldo/keto reductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.357; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.3.1.20

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; carboxylic acid binding; bile acid binding; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity

Biological Process: steroid metabolic process; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; epithelial cell differentiation; digestion; positive regulation of cell proliferation; progesterone metabolic process; prostaglandin metabolic process

Disease: 46,xy Sex Reversal 8