ENOX2 sirna

ENOX2 siRNA (Human)

Cat. Num. AAA8206634

• Gene Names: ENOX2; APK1; tNOX; COVA1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ENOX2; ENOX2 siRNA (Human); COVA1; Ecto-NOX disulfide-thiol exchanger 2; APK1 antigen; Cytosolic ovarian carcinoma antigen 1; Tumor-associated hydroquinone oxidase; tNOX) oxidase; Protein disulfide-thiol oxidoreductase ]; ENOX2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ENOX2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 581

• Applicable Applications for ENOX2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ENOX2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ENOX2 sirna

siRNA to inhibit ENOX2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 528524612
• NCBI GeneID: 10495
• NCBI Accession #: NP_001268665.1
• NCBI GenBank Nucleotide #: NM_001281736.1
• UniProt Accession #: Q16206; Q5VTJ1; Q5VTJ2; Q8WUX0; Q9NTP6; Q9UH82; A8K197; A8K1C2
• Molecular Weight: 66,621 Da
• NCBI Official Full Name: ecto-NOX disulfide-thiol exchanger 2 isoform a
• NCBI Official Synonym Full Names: ecto-NOX disulfide-thiol exchanger 2
• NCBI Official Symbol: ENOX2
• NCBI Official Synonym Symbols: APK1; tNOX; COVA1
• NCBI Protein Information: ecto-NOX disulfide-thiol exchanger 2
• UniProt Protein Name: Ecto-NOX disulfide-thiol exchanger 2
• Protein Family: Ecto-NOX disulfide-thiol exchanger
• UniProt Gene Name: ENOX2
• UniProt Synonym Gene Names: COVA1; tNOX
• UniProt Entry Name: ENOX2_HUMAN

NCBI Description

This gene is a tumor-specific member of the ECTO-NOX family of genes that encode cell surface NADH oxidases. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The protein also displays prion-like properties. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

ENOX2: May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide- thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock. Belongs to the ENOX family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.-.-.-; Cytoskeletal; Oxidoreductase; Cell surface

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: extracellular space; cytosol; external side of plasma membrane

Molecular Function: nucleic acid binding; nucleotide binding; protein disulfide oxidoreductase activity

Biological Process: regulation of growth; cell growth; ultradian rhythm