Western Blot (WB)
(Western blot analysis in Jurkat cell line lysates (35ug/lane) using MBS649724. This demonstrates that MBS649724 detected the ENOX2 protein (arrow).)
Rabbit anti-Human ENOX2 Polyclonal Antibody | anti-ENOX2 antibody
ENOX2, NT (ENOX2, COVA1, Ecto-NOX disulfide-thiol exchanger 2, APK1 antigen, Cytosolic ovarian carcinoma antigen 1, Tumor-associated hydroquinone oxidase, Hydroquinone [NADH] oxidase, Protein disulfide-thiol oxidoreductase)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western blot analysis in Jurkat cell line lysates (35ug/lane) using MBS649724. This demonstrates that MBS649724 detected the ENOX2 protein (arrow).)
Western Blot (WB)
(Western blot analysis in Jurkat cell line lysates (35ug/lane) using MBS649724. This demonstrates that MBS649724 detected the ENOX2 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue using MBS649724 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649724 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue using MBS649724 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649724 for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells using MBS649724 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells using MBS649724 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a tumor-specific member of the ECTO-NOX family of genes that encode cell surface NADH oxidases. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The protein also displays prion-like properties. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Uniprot Description
Function: May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock. Ref.10 Ref.15
Cofactor: Copper. Ref.1
Enzyme regulation: Inhibited by the antitumor sulfonylurea LY181984, the vabilloid capsaicin, and retinoids. Ref.1 Ref.9 Ref.10
Subcellular location: Cell membrane. Secreted › extracellular space. Note: Extracellular and plasma membrane-associated. Ref.8 Ref.10 Ref.13
Tissue specificity: Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed into serum by cancer cells. Found on the cell borders of renal, kidney and ovarian carcinomas but not on the borders of surrounding non-cancerous stromal cells.
Post-translational modification: Glycosylated. Ref.8
Miscellaneous: Has several properties associated with prions including resistance to proteases, resistance to cyanogen bromide digestion, and the ability to form amyloid filaments resembling those of spongiform encephalopathies.
Sequence similarities: Belongs to the ENOX family.Contains 1 RRM (RNA recognition motif) domain.
Sequence caution: The sequence AAB30428.1 differs from that shown. Reason: Frameshift at positions 302 and 357.
Research Articles on ENOX2
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Product Notes
The ENOX2 enox2 (Catalog #AAA649724) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENOX2, NT (ENOX2, COVA1, Ecto-NOX disulfide-thiol exchanger 2, APK1 antigen, Cytosolic ovarian carcinoma antigen 1, Tumor-associated hydroquinone oxidase, Hydroquinone [NADH] oxidase, Protein disulfide-thiol oxidoreductase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENOX2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the ENOX2 enox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENOX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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