Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) Standard Curve.)
Human Serine beta-Lactamase-Like Protein LACTB, Mitochondrial (LACTB) ELISA Kit | LACTB elisa kit
Human Serine beta-Lactamase-Like Protein LACTB, Mitochondrial (LACTB) ELISA Kit
Gene Names
LACTB; G24; MRPL56
Reactivity
Human
Synonyms
Serine beta-Lactamase-Like Protein LACTB; Mitochondrial (LACTB); Human Serine beta-Lactamase-Like Protein LACTB; Mitochondrial (LACTB) ELISA Kit; LACTB; FLJ14902; G24; MRPL56; mitochondrial 39S ribosomal protein L56; mitochondrial ribosomal protein L56; serine beta lactamase-like protein LACTB; LACTB elisa kit
Reactivity
Human
Specificity
Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) ELISA Kit has high sensitivity and excellent specificity for detection of Human LACTB. No significant cross-reactivity or interference between Human LACTB and analogues was observed.
Sequence Length
547
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) Standard Curve.)
Related Product Information for LACTB elisa kit
Description: This Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) ELISA Kit employs a two-site sandwich ELISA to quantitate LACTB in samples. An antibody specific for LACTB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LACTB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LACTB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LACTB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: LACTB encodes a protein from the large 39S subunit of the mitochondrial ribosome. LACTb has some sequence similarity to prokaryotic beta-lactamases but most of the residues that are responsible for the beta-lactamase activity are not conserved between the two proteins.The deduced 551-amino acid Lactb protein contains an N-terminal transmembrane domain and an alpha/beta-hydrolase fold catalytic site. LACTB, which encodes a predicted 549-amino acid protein that shares 90% amino acid identity with mouse Lactb. Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen. Northern blot analysis of human tissues detected a 2.3-kb transcript expressed at high levels in heart and skeletal muscle.
Background: LACTB encodes a protein from the large 39S subunit of the mitochondrial ribosome. LACTb has some sequence similarity to prokaryotic beta-lactamases but most of the residues that are responsible for the beta-lactamase activity are not conserved between the two proteins.The deduced 551-amino acid Lactb protein contains an N-terminal transmembrane domain and an alpha/beta-hydrolase fold catalytic site. LACTB, which encodes a predicted 549-amino acid protein that shares 90% amino acid identity with mouse Lactb. Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen. Northern blot analysis of human tissues detected a 2.3-kb transcript expressed at high levels in heart and skeletal muscle.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine beta-lactamase-like protein LACTB, mitochondrial isoform a
NCBI Official Synonym Full Names
lactamase beta
NCBI Official Symbol
LACTB
NCBI Official Synonym Symbols
G24; MRPL56
NCBI Protein Information
serine beta-lactamase-like protein LACTB, mitochondrial
UniProt Protein Name
Serine beta-lactamase-like protein LACTB, mitochondrial
Protein Family
UniProt Gene Name
LACTB
UniProt Synonym Gene Names
MRPL56
UniProt Entry Name
LACTB_HUMAN
NCBI Description
This gene encodes a mitochondrially-localized protein that has sequence similarity to prokaryotic beta-lactamases. Many of the residues responsible for beta-lactamase activity are not conserved in this protein, suggesting it may have a different enzymatic function. Increased expression of the related mouse gene was found to be associated with obesity. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Dec 2013]
Research Articles on LACTB
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Product Notes
The Human LACTB lactb (Catalog #AAA9715531) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715531 ELISA Kit recognizes Human LACTB. It is sometimes possible for the material contained within the vial of "Serine beta-Lactamase-Like Protein LACTB, Mitochondrial (LACTB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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