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Western Blot (WB) (ENOX2 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ENOX2 antibody detected the ENOX2 protein (arrow).)

Rabbit anti-Human ENOX2 Polyclonal Antibody | anti-ENOX2 antibody

ENOX2 Antibody (N-term)

Gene Names
ENOX2; APK1; tNOX; COVA1
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ENOX2; Polyclonal Antibody; ENOX2 Antibody (N-term); Ecto-NOX disulfide-thiol exchanger 2; APK1 antigen; Cytosolic ovarian carcinoma antigen 1; Tumor-associated hydroquinone oxidase; tNOX; Hydroquinone [NADH] oxidase; 1---; Protein disulfide-thiol oxidoreductase; COVA1; anti-ENOX2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ENOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ENOX2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.48 (varies by lot)
Sequence Positions
1-30
Sequence Length
581
Applicable Applications for anti-ENOX2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(ENOX2 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ENOX2 antibody detected the ENOX2 protein (arrow).)

Western Blot (WB) (ENOX2 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ENOX2 antibody detected the ENOX2 protein (arrow).)

Immunohistochemistry (IHC)

(ENOX2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENOX2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (ENOX2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENOX2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ENOX2 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ENOX2 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ENOX2 antibody
The protein encoded by this gene is a growth-related cell
surface protein. It was identifed because it reacts with the
monoclonal antibody KI in cells, such as the ovarian carcinoma line
OVCAR-3, also expressing the CAKI surface glycoprotein. The encoded
protein has two enzymatic activities: catalysis of hydroquinone or
NADH oxidation, and protein disulfide interchange. The two
activities alternate with a period length of about 24 minutes. The
encoded protein also displays prion-like properties. Two transcript
variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-ENOX2 antibody
References
Morre, D.M., et al. Rejuvenation Res 13 (2-3), 162-164 (2010) :
Morre, D.M., et al. Biofactors 34(3):237-244(2009)
Mao, L.C., et al. FEBS Lett. 582 (23-24), 3445-3450 (2008) :
Liu, S.C., et al. Biochem. Biophys. Res. Commun. 365(4):672-677(2008)
Tang, X., et al. Oncol. Res. 16(12):557-567(2007)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
70082
NCBI Official Full Name
ecto-NOX disulfide-thiol exchanger 2 isoform a
NCBI Official Synonym Full Names
ecto-NOX disulfide-thiol exchanger 2
NCBI Official Symbol
ENOX2
NCBI Official Synonym Symbols
APK1; tNOX; COVA1
NCBI Protein Information
ecto-NOX disulfide-thiol exchanger 2
UniProt Protein Name
Ecto-NOX disulfide-thiol exchanger 2
UniProt Gene Name
ENOX2
UniProt Synonym Gene Names
COVA1; tNOX
UniProt Entry Name
ENOX2_HUMAN

NCBI Description

This gene is a tumor-specific member of the ECTO-NOX family of genes that encode cell surface NADH oxidases. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The protein also displays prion-like properties. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

ENOX2: May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide- thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock. Belongs to the ENOX family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.-.-.-; Cell surface; Cytoskeletal

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: extracellular space; cytosol; external side of plasma membrane

Molecular Function: nucleic acid binding; nucleotide binding; protein disulfide oxidoreductase activity

Biological Process: regulation of growth; cell growth; ultradian rhythm

Research Articles on ENOX2

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Product Notes

The ENOX2 enox2 (Catalog #AAA9212518) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The ENOX2 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENOX2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the ENOX2 enox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENOX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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