EMX2 sirna

EMX2 siRNA (Human)

Cat. Num. AAA829742

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EMX2; EMX2 siRNA (Human); Homeobox protein EMX2; Empty spiracles homolog 2; Empty spiracles-like protein 2; EMX2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EMX2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 169

• Applicable Applications for EMX2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EMX2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EMX2 sirna

siRNA to inhibit EMX2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 260064072
• NCBI GeneID: 2018
• NCBI Accession #: NP_001159396.1
• NCBI GenBank Nucleotide #: NM_001165924.1
• UniProt Accession #: Q04743; Q96NN8; Q9BQF4; G3V305
• Molecular Weight: 18,518 Da
• NCBI Official Full Name: homeobox protein EMX2 isoform 2
• NCBI Official Synonym Full Names: empty spiracles homeobox 2
• NCBI Official Symbol: EMX2
• NCBI Protein Information: homeobox protein EMX2
• UniProt Protein Name: Homeobox protein EMX2
• Protein Family: Homeobox protein
• UniProt Gene Name: EMX2
• UniProt Entry Name: EMX2_HUMAN

NCBI Description

This gene encodes a homeobox-containing transcription factor that is the homolog to the 'empty spiracles' gene in Drosophila. Research on this gene in humans has focused on its expression in three tissues: dorsal telencephalon, olfactory neuroepithelium, and urogenetial system. It is expressed in the dorsal telencephalon during development in a low rostral-lateral to high caudal-medial gradient and is proposed to pattern the neocortex into defined functional areas. It is also expressed in embryonic and adult olfactory neuroepithelia where it complexes with eukaryotic translation initiation factor 4E (eIF4E) and possibly regulates mRNA transport or translation. In the developing urogenital system, it is expressed in epithelial tissues and is negatively regulated by HOXA10. Alternative splicing results in multiple transcript variants encoding distinct proteins.[provided by RefSeq, Sep 2009]

Uniprot Description

EMX2: Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system. Defects in EMX2 are the cause of schizencephaly (SCHZC). Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro- spinal fluid. Belongs to the EMX homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 10q26.1

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: forebrain cell migration; anterior/posterior pattern formation; neuron differentiation; response to drug; dentate gyrus development; regulation of transcription, DNA-dependent; cell proliferation in forebrain; cerebral cortex regionalization

Disease: Schizencephaly