DNAJA3 sirna

DNAJA3 siRNA (Human)

Cat. Num. AAA8210462

• Gene Names: DNAJA3; TID1; HCA57; hTID-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DNAJA3; DNAJA3 siRNA (Human); HCA57; TID1; DnaJ homolog subfamily A member 3 mitochondrial; DnaJ protein Tid-1; hTid-1; Hepatocellular carcinoma-associated antigen 57; Tumorous imaginal discs protein Tid56 homolog; DNAJA3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DNAJA3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 453

• Applicable Applications for DNAJA3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DNAJA3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DNAJA3 sirna

siRNA to inhibit DNAJA3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 205360840
• NCBI GeneID: 9093
• NCBI Accession #: NP_001128582.1
• NCBI GenBank Nucleotide #: NM_001135110.2
• UniProt Accession #: Q96EY1; O75472; Q8WUJ6; Q8WXJ3; Q96D76; Q96IV1; Q9NYH8; B2RAJ5; B4DI33; E7ES32
• Molecular Weight: 33,082 Da
• NCBI Official Full Name: dnaJ homolog subfamily A member 3, mitochondrial isoform 2
• NCBI Official Synonym Full Names: DnaJ (Hsp40) homolog, subfamily A, member 3
• NCBI Official Symbol: DNAJA3
• NCBI Official Synonym Symbols: TID1; HCA57; hTID-1
• NCBI Protein Information: dnaJ homolog subfamily A member 3, mitochondrial
• UniProt Protein Name: DnaJ homolog subfamily A member 3, mitochondrial
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: DNAJA3
• UniProt Synonym Gene Names: HCA57; TID1; hTid-1
• UniProt Entry Name: DNJA3_HUMAN

NCBI Description

This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

DNAJA3: Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma- mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway. Interacts with JAK2, HSPA9B and IFN-gammaR2 chain. Interacts with Ras GTPase-activating protein 1 (RASA1). Isoform 2 interacts with MUSK (via the cytoplasmic domain). Widely expressed with highest levels in heart, liver, lung and skeletal muscles. Also expressed in keratinocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell cycle regulation; Mitochondrial; Chaperone

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extrinsic to plasma membrane; postsynaptic membrane; intracellular membrane-bound organelle; mitochondrion; mitochondrial matrix; cytoplasm; actin filament; IkappaB kinase complex; cell junction; nucleus; cytosol; neuromuscular junction

Molecular Function: NF-kappaB binding; protein binding; chaperone binding; metal ion binding; unfolded protein binding; interferon-gamma receptor binding; Hsp70 protein binding; receptor tyrosine kinase binding; transcription factor binding; protein kinase binding; ATP binding

Biological Process: caspase activation; mitochondrion organization and biogenesis; protein folding; protein stabilization; positive regulation of apoptosis; T cell differentiation in the thymus; cell aging; negative regulation of transcription from RNA polymerase II promoter; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of caspase activity; negative regulation of cell proliferation; response to heat; inhibition of NF-kappaB transcription factor; regulation of catalytic activity; positive regulation of protein ubiquitination; small GTPase mediated signal transduction; negative regulation of programmed cell death; negative regulation of protein kinase activity; positive regulation of T cell proliferation; protein refolding; mitochondrial DNA replication; activated T cell apoptosis; neuromuscular junction development; negative regulation of apoptosis