Immunohistochemistry (IHC)
(Anti-PAG1 / PAG antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.)
Mouse PAG1 / PAG Monoclonal Antibody | anti-PAG1 antibody
Mouse Monoclonal [clone PAG-C1] (IgG2b) to Human PAG1 / PAG
Gene Names
PAG1; CBP; PAG
Reactivity
Bovine, Human, Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Protein A Purified
Synonyms
PAG1 / PAG; Monoclonal Antibody; Mouse Monoclonal [clone PAG-C1] (IgG2b) to Human PAG1 / PAG; Anti-PAG1 / PAG Antibody (C-Terminus; clone PAG-C1) IHC-plus; PAG1; Csk-binding protein; CBP; PAG; Human PAG1; anti-PAG1 antibody
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
PAG-C1
Specificity
Recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kD ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kD molecule.
Purity/Purification
Protein A Purified
Form/Format
PBS, 15 mM sodium azide, approx., pH 7.4.
Concentration
1 mg/ml (varies by lot)
Sequence Length
432
Applicable Applications for anti-PAG1 antibody
Immunohistochemistry (IHC - Paraffin), Immunoprecipitation (IP)
Application Notes
IHC-P (15 ug/ml), IP
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Immunogen Description
C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Immunogen Type
Synthetic peptide
Immunogen
PAG1 / PAG antibody was raised against c-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Antigen Modification
C-Terminus
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
(Anti-PAG1 / PAG antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.)
Immunohistochemistry (IHC)
(Anti-PAG1 / PAG antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.)
Immunoprecipitation (IP)
(Immunoprecipitation of human Cbp/PAG from the lysate of RAJI human Burkitt lymphoma cell line. An alternate antibody was used for detection of the immunoprecipitated protein.)
Immunoprecipitation (IP)
(Immunoprecipitation of human Cbp/PAG from the lysate of RAJI human Burkitt lymphoma cell line. An alternate antibody was used for detection of the immunoprecipitated protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,981 Da
NCBI Official Full Name
phosphoprotein associated with glycosphingolipid-enriched microdomains 1
NCBI Official Synonym Full Names
phosphoprotein membrane anchor with glycosphingolipid microdomains 1
NCBI Official Symbol
PAG1
NCBI Official Synonym Symbols
CBP; PAG
NCBI Protein Information
phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Csk-binding protein; transmembrane phosphoprotein Cbp; transmembrane adapter protein PAG; transmembrane adaptor protein PAG; phosphoprotein associated with glycosphingolipid microdomains 1
UniProt Protein Name
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
Protein Family
UniProt Gene Name
PAG1
UniProt Synonym Gene Names
CBP; PAG
UniProt Entry Name
PHAG1_HUMAN
NCBI Description
The protein encoded by this gene is a type III transmembrane adaptor protein that binds to the tyrosine kinase csk protein. It is thought to be involved in the regulation of T cell activation. [provided by RefSeq, Jul 2008]
Uniprot Description
PAG: an ubiquitous type III transmembrane adaptor protein that binds to the tyrosine kinase CSK protein. It is thought to be involved in the regulation of T cell activation.
Protein type: Membrane protein, integral; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 8q21.13
Cellular Component: integral to membrane; plasma membrane; lipid raft
Molecular Function: protein binding; SH3/SH2 adaptor activity; SH2 domain binding
Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of signal transduction; signal transduction; T cell receptor signaling pathway; negative regulation of T cell activation; regulation of T cell activation
Research Articles on PAG1
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Product Notes
The PAG1 pag1 (Catalog #AAA245321) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone PAG-C1] (IgG2b) to Human PAG1 / PAG reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PAG1 / PAG can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunoprecipitation (IP). IHC-P (15 ug/ml), IP Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the PAG1 pag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAG1 / PAG, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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