Typical Testing Data/Standard Curve (for reference only)
Mouse Glutamine synthetase ELISA Kit | GS elisa kit
Mouse GS (Glutamine synthetase) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GSand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GS.You can calculate the concentration of GSin the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Uniprot Description
GLUL: This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner. Essential for proliferation of fetal skin fibroblasts. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. Belongs to the glutamine synthetase family.
Protein type: EC 4.1.1.15; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Ligase; EC 6.3.1.2; Amino Acid Metabolism - arginine and proline
Chromosomal Location of Human Ortholog: 1q31
Cellular Component: cytoplasm; cytosol; mitochondrion; myelin sheath; nerve terminal; nucleus; perikaryon; protein complex; rough endoplasmic reticulum
Molecular Function: ATP binding; dynein light chain binding; glutamate binding; glutamate decarboxylase activity; glutamate-ammonia ligase activity; identical protein binding; magnesium ion binding; manganese ion binding
Biological Process: amino acid biosynthetic process; cell proliferation; cellular response to starvation; glutamate catabolic process; glutamine biosynthetic process; neurotransmitter uptake; positive regulation of epithelial cell proliferation; positive regulation of insulin secretion; positive regulation of synaptic transmission, glutamatergic; protein homooligomerization; response to glucose stimulus; synaptic transmission
Disease: Glutamine Deficiency, Congenital
Research Articles on GS
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Product Notes
The Mouse GS glul (Catalog #AAA2533465) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533465 ELISA Kit recognizes Mouse GS. It is sometimes possible for the material contained within the vial of "Glutamine synthetase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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