Western Blot (WB)
(Western blot analysis of DNAJA3 expression in Jurkat (A), HepG2 (B), mouse kidney (C), mouse heart (D), rat liver (E) whole cell lysates.)
Rabbit DNAJA3 Polyclonal Antibody | anti-DNAJA3 antibody
Anti-DNAJA3 Antibody
Western Blot (WB)
(Western blot analysis of DNAJA3 expression in Jurkat (A), HepG2 (B), mouse kidney (C), mouse heart (D), rat liver (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of DNAJA3 expression in Jurkat (A), HepG2 (B), mouse kidney (C), mouse heart (D), rat liver (E) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of DNAJA3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of DNAJA3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
DNAJA3: Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma- mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway. Interacts with JAK2, HSPA9B and IFN-gammaR2 chain. Interacts with Ras GTPase-activating protein 1 (RASA1). Isoform 2 interacts with MUSK (via the cytoplasmic domain). Widely expressed with highest levels in heart, liver, lung and skeletal muscles. Also expressed in keratinocytes. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; Mitochondrial; Chaperone; Cell cycle regulation
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: actin filament; cell junction; cytoplasm; cytosol; extrinsic to plasma membrane; IkappaB kinase complex; intracellular membrane-bound organelle; mitochondrial matrix; mitochondrion; neuromuscular junction; nucleus; postsynaptic membrane
Molecular Function: ATP binding; GTPase regulator activity; Hsp70 protein binding; interferon-gamma receptor binding; metal ion binding; NF-kappaB binding; protein binding; protein kinase binding; receptor tyrosine kinase binding; transcription factor binding; unfolded protein binding
Biological Process: activated T cell apoptosis; caspase activation; cell aging; inhibition of NF-kappaB transcription factor; mitochondrial DNA replication; mitochondrion organization and biogenesis; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of cell proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of programmed cell death; negative regulation of protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; neuromuscular junction development; positive regulation of apoptosis; positive regulation of protein ubiquitination; positive regulation of T cell proliferation; protein folding; protein stabilization; regulation of catalytic activity; response to heat; small GTPase mediated signal transduction; T cell differentiation in the thymus
Research Articles on DNAJA3
Similar Products
Product Notes
The DNAJA3 dnaja3 (Catalog #AAA8245255) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DNAJA3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/100. Researchers should empirically determine the suitability of the DNAJA3 dnaja3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
