DLGAP5 sirna

DLGAP5 siRNA (Human)

Cat. Num. AAA8220417

• Gene Names: DLGAP5; DLG7; HURP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DLGAP5; DLGAP5 siRNA (Human); DLG7; KIAA0008; Disks large-associated protein 5; DAP-5; Discs large homolog 7; Disks large-associated protein DLG7; Hepatoma up-regulated protein; HURP; DLGAP5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DLGAP5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 842

• Applicable Applications for DLGAP5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DLGAP5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DLGAP5 sirna

siRNA to inhibit DLGAP5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226371669
• NCBI GeneID: 9787
• NCBI Accession #: NP_001139487.1
• NCBI GenBank Nucleotide #: NM_001146015.1
• UniProt Accession #: Q15398; Q86T11; Q8NG58; A8MTM6; B4DRM8
• Molecular Weight: 94,178 Da
• NCBI Official Full Name: disks large-associated protein 5 isoform b
• NCBI Official Synonym Full Names: discs, large (Drosophila) homolog-associated protein 5
• NCBI Official Symbol: DLGAP5
• NCBI Official Synonym Symbols: DLG7; HURP
• NCBI Protein Information: disks large-associated protein 5
• UniProt Protein Name: Disks large-associated protein 5
• Protein Family: Disks large-associated protein
• UniProt Gene Name: DLGAP5
• UniProt Synonym Gene Names: DLG7; KIAA0008; DAP-5; HURP
• UniProt Entry Name: DLGP5_HUMAN

Uniprot Description

DLG7: a cell cycle regulator that may play a role in carcinogenesis. Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells. Required for chromosome congression and alignment. Stabilizes mitotic microtubules, promotes microtubule polymerization and controls spindle formation and dynamics Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Interacts with CDC2 and localizes to the spindle poles in mitotic cells. Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDC2/Cyclin B-phosphorylation dependent manner. Colocalizes with E-cadherin (CDH1) at sites of cell-cell contact in intestinal epithelial cells. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells. Two alternatively spliced isoforms have been described.

Protein type: Microtubule-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: cytoplasm; microtubule organizing center; spindle pole centrosome; nucleus

Molecular Function: protein binding; phosphoprotein phosphatase activity

Biological Process: cell proliferation; cell-cell signaling; dephosphorylation; positive regulation of mitotic metaphase/anaphase transition; mitotic chromosome movement towards spindle pole