Mouse Eukaryotic translation initiation factor 2 subunit 1 (EIF2S1) ELISA Kit | EIF2S1 elisa kit

Mouse Eukaryotic translation initiation factor 2 subunit 1 (EIF2S1) ELISA Kit

Cat. Num. AAA7212152

• Gene Names: EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
• Reactivity: Mouse
• Synonyms: Eukaryotic translation initiation factor 2 subunit 1 (EIF2S1); Mouse Eukaryotic translation initiation factor 2 subunit 1 (EIF2S1) ELISA Kit; EIF2S1 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This EIF2S1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse EIF2S1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EIF2S1 elisa kit

Principle of the assay: EIF2S1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EIF2S1 antibody and an EIF2S1-HRP conjugate. The assay sample and buffer are incubated together with EIF2S1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EIF2S1 concentration since EIF2S1 from samples and EIF2S1-HRP conjugate compete for the anti-EIF2S1 antibody binding site. Since the number of sites is limited, as more sites are occupied by EIF2S1 from the sample, fewer sites are left to bind EIF2S1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EIF2S1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for EIF2S1 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4758256
• NCBI GeneID: 1965
• NCBI Accession #: NP_004085.1
• NCBI GenBank Nucleotide #: NM_004094.4
• Molecular Weight: 36,112 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 2 subunit 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• NCBI Official Symbol: EIF2S1
• NCBI Official Synonym Symbols: EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
• NCBI Protein Information: eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha
• UniProt Protein Name: Eukaryotic translation initiation factor 2 subunit 1
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF2S1
• UniProt Synonym Gene Names: EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha
• UniProt Entry Name: IF2A_HUMAN

NCBI Description

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Uniprot Description

eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.

Protein type: RNA-binding; Translation; Translation initiation

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; cytosol; nucleus; eukaryotic translation initiation factor 2 complex

Molecular Function: protein binding; translation initiation factor activity; ribosome binding

Biological Process: regulation of translation initiation in response to stress; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression