DAB2 sirna

DAB2 siRNA (Human)

Cat. Num. AAA8204275

• Gene Names: DAB2; DOC2; DOC-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DAB2; DAB2 siRNA (Human); DOC2; Disabled homolog 2; DOC-2; Differentially-expressed protein 2; DAB2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DAB2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 749

• Applicable Applications for DAB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DAB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DAB2 sirna

siRNA to inhibit DAB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 349585060
• NCBI GeneID: 1601
• NCBI Accession #: NP_001231800.1
• NCBI GenBank Nucleotide #: NM_001244871.1
• UniProt Accession #: P98082; Q13598; Q9BTY0; Q9UK04; A6NES5
• Molecular Weight: 80,228 Da
• NCBI Official Full Name: disabled homolog 2 isoform 2
• NCBI Official Synonym Full Names: Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila)
• NCBI Official Symbol: DAB2
• NCBI Official Synonym Symbols: DOC2; DOC-2
• NCBI Protein Information: disabled homolog 2
• UniProt Protein Name: Disabled homolog 2
• Protein Family: Disabled
• UniProt Gene Name: DAB2
• UniProt Synonym Gene Names: DOC2; DOC-2
• UniProt Entry Name: DAB2_HUMAN

NCBI Description

This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

DAB2: an adaptor protein that interacts with Grb2, myosin VI, SMAD2/3, DIP1/2, Dvl-3, the integrin betasubunit, and c-Src. Negatively regulates canonical Wnt signaling by stabilizing the beta-catenin degradation complex. A component of the CSF-1 signal transduction pathway. Phosphorylated DAB2 inhibits adhesion and integrin signaling. Two alternatively spliced isoforms are described.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: focal adhesion; intracellular membrane-bound organelle; clathrin-coated vesicle; lysosomal membrane; apical plasma membrane; nucleolus; plasma membrane; clathrin coated vesicle membrane; coated pit; clathrin coat of coated pit

Molecular Function: integrin binding; protein C-terminus binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; SMAD binding

Biological Process: cellular morphogenesis during differentiation; pinocytosis; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of endocytosis; excretion; activation of JNK activity; clathrin cage assembly; protein transport; positive regulation of RNA elongation from RNA polymerase II promoter; negative regulation of protein binding; positive regulation of receptor recycling; integrin-mediated signaling pathway; receptor-mediated endocytosis; Wnt receptor signaling pathway; in utero embryonic development; leading edge cell differentiation; positive regulation of transforming growth factor beta receptor signaling pathway; cell proliferation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; myeloid cell differentiation; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; endoderm development; positive regulation of cell migration