CLCA2 sirna

CLCA2 siRNA (Human)

Cat. Num. AAA8202243

• Gene Names: CLCA2; CACC; CACC3; CLCRG2; CaCC-3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CLCA2; CLCA2 siRNA (Human); CACC3; Calcium-activated chloride channel regulator 2; Calcium-activated chloride channel family member 2; hCLCA2; Calcium-activated chloride channel protein 3; CaCC-3; hCaCC-3; CLCA2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CLCA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 943

• Applicable Applications for CLCA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CLCA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CLCA2 sirna

siRNA to inhibit CLCA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5729769
• NCBI GeneID: 9635
• NCBI Accession #: NP_006527.1
• NCBI GenBank Nucleotide #: NM_006536.5
• UniProt Accession #: Q9UQC9; Q9Y6N2; A8K2T3
• Molecular Weight: 103,941 Da
• NCBI Official Full Name: calcium-activated chloride channel regulator 2
• NCBI Official Synonym Full Names: chloride channel accessory 2
• NCBI Official Symbol: CLCA2
• NCBI Official Synonym Symbols: CACC; CACC3; CLCRG2; CaCC-3
• NCBI Protein Information: calcium-activated chloride channel regulator 2
• UniProt Protein Name: Calcium-activated chloride channel regulator 2
• Protein Family: Calcium-activated chloride channel regulator
• UniProt Gene Name: CLCA2
• UniProt Synonym Gene Names: CACC3; hCLCA2; CaCC-3; hCaCC-3
• UniProt Entry Name: CLCA2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same site on chromosome 1p31-p22 and share high degrees of homology in size, sequence and predicted structure, but differ significantly in their tissue distributions. Since this protein is expressed predominantly in trachea and lung, it is suggested to play a role in the complex pathogenesis of cystic fibrosis. It may also serve as adhesion molecule for lung metastatic cancer cells, mediating vascular arrest and colonization, and furthermore, it has been implicated to act as a tumor suppressor gene for breast cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

CLCA5: Plays a role in modulating chloride current across the plasma membrane in a calcium-dependent manner, and cell adhesion. Involved in basal cell adhesion and/or stratification of squamous epithelia. May act as a tumor suppressor in breast and colorectal cancer. Plays a key role for cell adhesion in the beginning stages of lung metastasis via the binding to ITGB4. Belongs to the CLCR family.

Protein type: Channel, chloride; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; basal plasma membrane; cell junction

Molecular Function: chloride channel activity; metallopeptidase activity; metal ion binding; ligand-gated ion channel activity

Biological Process: transport; proteolysis; cell adhesion; transmembrane transport