CHRNB3 sirna

CHRNB3 siRNA (Human)

Cat. Num. AAA8226821

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHRNB3; CHRNB3 siRNA (Human); Neuronal acetylcholine receptor subunit beta-3; CHRNB3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CHRNB3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 458

• Applicable Applications for CHRNB3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CHRNB3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHRNB3 sirna

siRNA to inhibit CHRNB3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4502835
• NCBI GeneID: 1142
• NCBI Accession #: NP_000740.1
• NCBI GenBank Nucleotide #: NM_000749.3
• UniProt Accession #: Q05901; Q15827
• Molecular Weight: 52,729 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit beta-3
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, beta 3 (neuronal)
• NCBI Official Symbol: CHRNB3
• NCBI Protein Information: neuronal acetylcholine receptor subunit beta-3
• UniProt Protein Name: Neuronal acetylcholine receptor subunit beta-3
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNB3
• UniProt Entry Name: ACHB3_HUMAN

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.[supplied by OMIM, Apr 2010]

Uniprot Description

nAChRB3: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 3/CHRNB3 sub-subfamily.

Protein type: Channel, cation; Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; neuron projection; plasma membrane; integral to membrane; cell junction

Molecular Function: channel activity; drug binding; acetylcholine binding; nicotinic acetylcholine-activated cation-selective channel activity

Biological Process: synaptic transmission; protein heterooligomerization; transport; signal transduction; transmembrane transport; cation transport; synaptic transmission, cholinergic