CHRNB3 recombinant protein

CHRNB3 Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8121857

• Purity: >85% as determined by SDS-PAGE
• Synonyms: CHRNB3; CHRNB3 Protein; Human; Recombinant (ECD; hFc Tag); Human CHRNB3 Protein (Fc Tag; ECD); cholinergic receptor; nicotinic; beta 3 (neuronal); CHRNB3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu232

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Ile 25
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human CHRNB3 (NP_000740.1)(Met1-Leu232) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CHRNB3 recombinant protein

Background: Cholinergic nicotinic receptor (CHRN) gene family has been known to mediate the highly additive effects of nicotine in the body, and implicated nicotine dependence (ND) and related phenotypes. Cigarette smoking is protective in Parkinson's disease (PD), possibly because of nicotine action on brain nicotinic-acetylcholine receptors. The beta3 nicotinic-acetylcholine receptor subunit (encoded by CHRNB3) is depleted in the striatum of PD patients and associated with nicotine dependence.

NCBI and Uniprot Product Information

• NCBI GI #: 4502835
• NCBI GeneID: 1142
• NCBI Accession #: NP_000740.1
• NCBI GenBank Nucleotide #: NM_000749.3
• Molecular Weight: 52,729 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit beta-3
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic beta 3 subunit
• NCBI Official Symbol: CHRNB3
• NCBI Protein Information: neuronal acetylcholine receptor subunit beta-3
• Protein Family: Neuronal acetylcholine receptor

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.[supplied by OMIM, Apr 2010]