Western Blot (WB)
(FBXW12 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the FBXW12 antibody detected the FBXW12 protein (arrow).)
Rabbit anti-Human FBXW12 Polyclonal Antibody | anti-FBXW12 antibody
FBXW12, CT (FBXW12, FBW12, FBXO12, FBXO35, F-box/WD repeat-containing protein 12, F-box and WD-40 domain-containing protein 12, F-box only protein 35) (PE)
Gene Names
FBXW12; FBW12; FBXO12; FBXO35
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
FBXW12; Polyclonal Antibody; CT (FBXW12; FBW12; FBXO12; FBXO35; F-box/WD repeat-containing protein 12; F-box and WD-40 domain-containing protein 12; F-box only protein 35) (PE); F-box only protein 35; anti-FBXW12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-FBXW12 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
FBXW12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-328 amino acids from the C-terminal region of human FBXW12.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(FBXW12 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the FBXW12 antibody detected the FBXW12 protein (arrow).)
Western Blot (WB)
(FBXW12 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the FBXW12 antibody detected the FBXW12 protein (arrow).)
Immunohistochemistry (IHC)
(FBXW12 antibody(C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXW12 antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(FBXW12 antibody(C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXW12 antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(FBXW12 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(FBXW12 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-FBXW12 antibody
Members of the F-box protein family, such as FBXW12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603034), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM].
Product Categories/Family for anti-FBXW12 antibody
References
Jin, J., et al. Genes Dev. 18(21):2573-2580(2004). Zeng, L., et al. Mol. Biol. Rep. 31(1):51-57(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,056 Da
NCBI Official Full Name
F-box/WD repeat-containing protein 12 isoform 2
NCBI Official Synonym Full Names
F-box and WD repeat domain containing 12
NCBI Official Symbol
FBXW12
NCBI Official Synonym Symbols
FBW12; FBXO12; FBXO35
NCBI Protein Information
F-box/WD repeat-containing protein 12; F-box only protein 35; F-box and WD-40 domain protein 12; F-box- and WD40-repeat-containing protein; F-box and WD-40 domain-containing protein 12
UniProt Protein Name
F-box/WD repeat-containing protein 12
Protein Family
UniProt Gene Name
FBXW12
UniProt Synonym Gene Names
FBW12; FBXO12; FBXO35
UniProt Entry Name
FBW12_HUMAN
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Product Notes
The FBXW12 fbxw12 (Catalog #AAA6298537) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FBXW12, CT (FBXW12, FBW12, FBXO12, FBXO35, F-box/WD repeat-containing protein 12, F-box and WD-40 domain-containing protein 12, F-box only protein 35) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FBXW12 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the FBXW12 fbxw12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FBXW12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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