CAPN2 sirna

CAPN2 siRNA (Human)

Cat. Num. AAA8229957

• Gene Names: CAPN2; CANP2; mCANP; CANPL2; CANPml
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CAPN2; CAPN2 siRNA (Human); CANPL2; Calpain-2 catalytic subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain; CAPN2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CAPN2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 622

• Applicable Applications for CAPN2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CAPN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CAPN2 sirna

siRNA to inhibit CAPN2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 225703100
• NCBI GeneID: 824
• NCBI Accession #: NP_001139540.1
• NCBI GenBank Nucleotide #: NM_001146068.1
• UniProt Accession #: P17655; Q16738; Q6PJT3; Q8WU26; Q9HBB1; A6NDG7; B7ZA96; E7ES58
• Molecular Weight: 71,476 Da
• NCBI Official Full Name: calpain-2 catalytic subunit isoform 2
• NCBI Official Synonym Full Names: calpain 2, (m/II) large subunit
• NCBI Official Symbol: CAPN2
• NCBI Official Synonym Symbols: CANP2; mCANP; CANPL2; CANPml
• NCBI Protein Information: calpain-2 catalytic subunit
• UniProt Protein Name: Calpain-2 catalytic subunit
• Protein Family: Calpain
• UniProt Gene Name: CAPN2
• UniProt Synonym Gene Names: CANPL2; CANP 2; M-calpain
• UniProt Entry Name: CAN2_HUMAN

NCBI Description

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

CAPN2: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Protease; EC 3.4.22.53

Chromosomal Location of Human Ortholog: 1q41-q42

Cellular Component: Golgi apparatus; cortical actin cytoskeleton; focal adhesion; endoplasmic reticulum; dendrite; cytoplasm; plasma membrane; pseudopodium; chromatin; nucleus; cytosol; lipid raft

Molecular Function: protein binding; protein heterodimerization activity; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; cysteine-type peptidase activity

Biological Process: protein autoprocessing; blastocyst development; proteolysis involved in cellular protein catabolic process; response to hypoxia; proteolysis; myoblast fusion; regulation of cytoskeleton organization and biogenesis