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Western Blot (WB) (Western Blot analysis of Mouse skin tissue using EIF3F Polyclonal Antibody at dilution of 1:250)

Rabbit EIF3F Polyclonal Antibody | anti-EIF3F antibody

EIF3F Polyclonal Antibody

Gene Names
EIF3F; EIF3S5; eIF3-p47
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
EIF3F; Polyclonal Antibody; EIF3F Polyclonal Antibody; EIF3S5; eIF3-p47; anti-EIF3F antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
1.3mg/mL (varies by lot)
Sequence Length
357
Applicable Applications for anti-EIF3F antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:200-1:1000
IHC: 1:50-1:200
Immunogen
Recombinant protein of human EIF3F
Buffer
PBS with 0.05% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Mouse skin tissue using EIF3F Polyclonal Antibody at dilution of 1:250)

Western Blot (WB) (Western Blot analysis of Mouse skin tissue using EIF3F Polyclonal Antibody at dilution of 1:250)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human colon cancer using EIF3F Polyclonal Antibody at dilution of 1:30)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human colon cancer using EIF3F Polyclonal Antibody at dilution of 1:30)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer using EIF3F Polyclonal Antibody at dilution of 1:30)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using EIF3F Polyclonal Antibody at dilution of 1:30)
Related Product Information for anti-EIF3F antibody
Eukaryotic translation initiation factor 3 subunit F is a protein that in humans is encoded by the EIF3F gene. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,564 Da
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit F
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3, subunit F
NCBI Official Symbol
EIF3F
NCBI Official Synonym Symbols
EIF3S5; eIF3-p47
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit F; EIF3F; deubiquitinating enzyme eIF3f; eIF-3-epsilon; eIF3-epsilon; eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD); eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit F
UniProt Gene Name
EIF3F
UniProt Entry Name
EIF3F_HUMAN

Uniprot Description

eIF3-epsilon: eukaryotic translation initiation factor 3, subunit 5 epsilon. Binds to the 40s ribosome and promotes the binding of methionyl-tRNAi and mRNA. Associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits.

Protein type: EC 3.4.19.12; Translation initiation; Translation

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: eukaryotic translation initiation factor 3 complex; membrane; cytosol

Molecular Function: protein binding; translation initiation factor binding; translation initiation factor activity; ubiquitin-specific protease activity

Biological Process: cellular protein metabolic process; protein deubiquitination; translation; translational initiation; gene expression; proteolysis; formation of translation preinitiation complex; regulation of translational initiation

Research Articles on EIF3F

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Product Notes

The EIF3F eif3f (Catalog #AAA2527559) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF3F Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3F can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:200-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the EIF3F eif3f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF3F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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