BTAF1 sirna

BTAF1 siRNA (Human)

Cat. Num. AAA8215471

• Gene Names: BTAF1; MOT1; TAF172; TAFII170; TAF(II)170
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BTAF1; BTAF1 siRNA (Human); TAF172; TATA-binding protein-associated factor 172; ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; TAF(II)170; TBP-associated factor 172; TAF-172; BTAF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: BTAF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1849

• Applicable Applications for BTAF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BTAF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BTAF1 sirna

siRNA to inhibit BTAF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 27477070
• NCBI GeneID: 9044
• NCBI Accession #: NP_003963.1
• NCBI GenBank Nucleotide #: NM_003972.2
• UniProt Accession #: O14981; O43578; B4E0W6
• Molecular Weight: 76,419 Da
• NCBI Official Full Name: TATA-binding protein-associated factor 172
• NCBI Official Synonym Full Names: BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa
• NCBI Official Symbol: BTAF1
• NCBI Official Synonym Symbols: MOT1; TAF172; TAFII170; TAF(II)170
• NCBI Protein Information: TATA-binding protein-associated factor 172
• UniProt Protein Name: TATA-binding protein-associated factor 172
• Protein Family: TATA-binding protein-associated factor
• UniProt Gene Name: BTAF1
• UniProt Synonym Gene Names: TAF172; TAF-172
• UniProt Entry Name: BTAF1_HUMAN

NCBI Description

This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters. [provided by RefSeq, Sep 2011]

Uniprot Description

TAF172: Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner. Associates with TBP to form B-TFIID. Binds DRAP1. Belongs to the SNF2/RAD54 helicase family.

Protein type: EC 3.6.1.-; EC 3.6.4.-; Transcription initiation complex; Helicase

Chromosomal Location of Human Ortholog: 10q22-q23

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity; helicase activity; ATP binding

Biological Process: metabolic process; negative regulation of transcription, DNA-dependent