Rabbit anti-Human, Mouse GITRL Polyclonal Antibody | anti-TNFSF18 antibody

GITRL Antibody

Cat. Num. AAA150890

• Gene Names: TNFSF18; TL6; AITRL; GITRL; hGITRL
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: GITRL Antibody is affinity chromatography purified via peptide column.
• Synonyms: GITRL; Polyclonal Antibody; GITRL Antibody; TL6; AITRL; hGITRL; UNQ149/PRO175; Tumor necrosis factor ligand superfamily member 18; Activation-inducible TNF-related ligand; tumor necrosis factor (ligand) superfamily; member 18; anti-TNFSF18 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: GITRL Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 177

• Applicable Applications for anti-TNFSF18 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: GITRL antibody can be used for the detection of GITRL by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: GITRL antibody was raised against purified recombinant human GITR ligand.
• Buffer: GITRL Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: GITRL antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of (A) 5 ng, (B) 25 ng, and (C) 50 ng of purified recombinant GITRL with ITRL antibody at 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of GITRL in THP-1 cells with GITRL antibody at 10 μg/mL.)

Related Product Information for anti-TNFSF18 antibody

GITRL Antibody: The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-κB and various mitogen-activated protein kinases. The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions. Its ligand GITRL is expressed in endothelial and antigen-presenting cells and can activate NF-κB, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR. Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-κB activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.

NCBI and Uniprot Product Information

• NCBI GI #: 13124621
• NCBI GeneID: 8995
• UniProt Accession #: Q9UNG2; O95852; Q6ISV1; A9IQG8
• Molecular Weight: 22,724 Da
• NCBI Official Full Name: Tumor necrosis factor ligand superfamily member 18
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 18
• NCBI Official Symbol: TNFSF18
• NCBI Official Synonym Symbols: TL6; AITRL; GITRL; hGITRL
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 18; AITR ligand; GITR ligand; activation-inducible TNF-related ligand; glucocorticoid-induced TNF-related ligand; glucocorticoid-induced TNFR-related protein ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 18
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF18
• UniProt Synonym Gene Names: AITRL; GITRL; TL6; AITRL; hGITRL
• UniProt Entry Name: TNF18_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFSF18: Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T- cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: extracellular space; cell surface; integral to membrane; plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding; receptor binding

Biological Process: tumor necrosis factor-mediated signaling pathway; cell-cell signaling; T cell proliferation during immune response; regulation of T cell proliferation; signal transduction; activation of NF-kappaB transcription factor; negative regulation of apoptosis