ARPP19 sirna

ARPP19 siRNA (Human)

Cat. Num. AAA8218908

• Gene Names: ARPP19; ENSAL; ARPP16; ARPP-16; ARPP-19
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARPP19; ARPP19 siRNA (Human); cAMP-regulated phosphoprotein 19; ARPP-19; ARPP19 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ARPP19 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 131

• Applicable Applications for ARPP19 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARPP19 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARPP19 sirna

siRNA to inhibit ARPP19 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 807201093
• NCBI GeneID: 10776
• NCBI Accession #: NP_001293120.1
• NCBI GenBank Nucleotide #: NM_001306191.1
• UniProt Accession #: P56211; Q6IAM2; Q86TA6; Q9UD70; B2R497
• Molecular Weight: 10,635 Da
• NCBI Official Full Name: cAMP-regulated phosphoprotein 19 isoform 1
• NCBI Official Synonym Full Names: cAMP-regulated phosphoprotein, 19kDa
• NCBI Official Symbol: ARPP19
• NCBI Official Synonym Symbols: ENSAL; ARPP16; ARPP-16; ARPP-19
• NCBI Protein Information: cAMP-regulated phosphoprotein 19
• UniProt Protein Name: cAMP-regulated phosphoprotein 19
• Protein Family: cAMP-regulated phosphoprotein
• UniProt Gene Name: ARPP19
• UniProt Synonym Gene Names: ARPP-19
• UniProt Entry Name: ARP19_HUMAN

NCBI Description

The 19-kD cAMP-regulated phosphoprotein plays a role in regulating mitosis by inhibiting protein phosphatase-2A (PP2A; see MIM 176915) (summary by Gharbi-Ayachi et al., 2010 [PubMed 21164014]).[supplied by OMIM, Feb 2011]

Uniprot Description

ARPP-19: a protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at S62 by MASTL during mitosis, specifically interacts with PPP2R2D and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. May indirectly enhance GAP43 expression. Interacts with SNCA. Two isoforms of the human protein, ARPP-19 and ARPP-16, are produced by alternative splicing.

Protein type: Phosphatase, regulatory subunit; Cell cycle regulation

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: phosphatase inhibitor activity; potassium channel regulator activity; protein phosphatase 2A binding; protein phosphatase type 2A regulator activity; protein phosphatase inhibitor activity; receptor binding

Biological Process: positive regulation of glucose import; mitosis; positive regulation of gluconeogenesis; cell division; regulation of catalytic activity; negative regulation of catalytic activity; mitotic cell cycle; G2/M transition of mitotic cell cycle