Goat Fc Fragment Of IgA Receptor ELISA Kit | FCAR elisa kit

Goat Fc Fragment Of IgA Receptor ELISA Kit

Cat. Num. AAA735307

• Reactivity: Goat
• Synonyms: Fc Fragment Of IgA Receptor; Goat Fc Fragment Of IgA Receptor ELISA Kit; FCAR elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This FCAR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat FCAR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FCAR elisa kit

Principle of the assay: FCAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FCAR antibody and an FCAR-HRP conjugate. The assay sample and buffer are incubated together with FCAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FCAR concentration since FCAR from samples and FCAR-HRP conjugate compete for the anti-FCAR antibody binding site. Since the number of sites is limited, as more sites are occupied by FCAR from the sample, fewer sites are left to bind FCAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FCAR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FCAR elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 129447255
• Molecular Weight: 11,293 Da
• NCBI Official Full Name: FCAR, partial
• UniProt Protein Name: FCAR
• Protein Family: Immunoglobulin alpha Fc receptor
• UniProt Gene Name: FCAR
• UniProt Entry Name: A5Y2F0_HUMAN

NCBI Description

This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FCAR: Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: IgA binding

Biological Process: immune response