Western Blot (WB)
(The anti-PDAP1 Antibody (C-term) is used in Western blot to detect PDAP1 in HL60 lysate.)
Rabbit anti-Human PDAP1 Polyclonal Antibody | anti-PDAP1 antibody
PDAP1, CT (28kD Heat-and Acid-stable Phosphoprotein, PDGF-associated Protein, PAP, PDGFA-associated Protein 1, PAP1, HASPP28) (Azide free) (HRP)
Gene Names
PDAP1; PAP; PAP1; HASPP28
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
PDAP1; Polyclonal Antibody; CT (28kD Heat-and Acid-stable Phosphoprotein; PDGF-associated Protein; PAP; PDGFA-associated Protein 1; PAP1; HASPP28) (Azide free) (HRP); anti-PDAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PDAP1.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-PDAP1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human PDAP1 (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(The anti-PDAP1 Antibody (C-term) is used in Western blot to detect PDAP1 in HL60 lysate.)
Western Blot (WB)
(The anti-PDAP1 Antibody (C-term) is used in Western blot to detect PDAP1 in HL60 lysate.)
Western Blot (WB)
(Western blot analysis of PDAP1(arrow) using rabbit polyclonal PDAP1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDAP1 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of PDAP1(arrow) using rabbit polyclonal PDAP1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDAP1 gene (Lane 2) (Origene Technologies).)
Product Categories/Family for anti-PDAP1 antibody
References
1. Fischer, W.H., et al., J. Neurochem. 66(5):2213-2216 (1996).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,630 Da
NCBI Official Full Name
28 kDa heat- and acid-stable phosphoprotein
NCBI Official Synonym Full Names
PDGFA associated protein 1
NCBI Official Symbol
PDAP1
NCBI Official Synonym Symbols
PAP; PAP1; HASPP28
NCBI Protein Information
28 kDa heat- and acid-stable phosphoprotein; OTTHUMP00000206311; PDGF-associated protein
UniProt Protein Name
28 kDa heat- and acid-stable phosphoprotein
Protein Family
UniProt Gene Name
PDAP1
UniProt Synonym Gene Names
HASPP28
UniProt Entry Name
HAP28_HUMAN
Similar Products
Product Notes
The PDAP1 pdap1 (Catalog #AAA6492440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDAP1, CT (28kD Heat-and Acid-stable Phosphoprotein, PDGF-associated Protein, PAP, PDGFA-associated Protein 1, PAP1, HASPP28) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PDAP1 pdap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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