ARID5B sirna

ARID5B siRNA (Human)

Cat. Num. AAA8240418

• Gene Names: ARID5B; MRF2; DESRT; MRF-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARID5B; ARID5B siRNA (Human); DESRT; MRF2; AT-rich interactive domain-containing protein 5B; ARID domain-containing protein 5B; MRF1-like protein; Modulator recognition factor 2; MRF-2; ARID5B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ARID5B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 945

• Applicable Applications for ARID5B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARID5B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARID5B sirna

siRNA to inhibit ARID5B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 347582593
• NCBI GeneID: 84159
• NCBI Accession #: NP_001231567.1
• NCBI GenBank Nucleotide #: NM_001244638.1
• UniProt Accession #: Q14865; Q05DG6; Q32Q59; Q5VST4; Q6NZ42; Q7Z3M4; Q8N421; Q9H786; B4DLB3
• Molecular Weight: 36,282 Da
• NCBI Official Full Name: AT-rich interactive domain-containing protein 5B isoform 2
• NCBI Official Synonym Full Names: AT rich interactive domain 5B (MRF1-like)
• NCBI Official Symbol: ARID5B
• NCBI Official Synonym Symbols: MRF2; DESRT; MRF-2
• NCBI Protein Information: AT-rich interactive domain-containing protein 5B
• UniProt Protein Name: AT-rich interactive domain-containing protein 5B
• Protein Family: AT-rich interactive domain-containing protein
• UniProt Gene Name: ARID5B
• UniProt Synonym Gene Names: DESRT; MRF2; ARID domain-containing protein 5B; MRF-2
• UniProt Entry Name: ARI5B_HUMAN

NCBI Description

This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

Mrf-2: Transcription coactivator that binds to the 5'-AATA[CT]- 3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2- ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. Defects in ARID5B may be a cause of susceptibility to coronary atherosclerosis in the Japanese population. Defects in ARID5B may be a cause of susceptibility to acute lymphoblastic leukemia (ALL). ALL is a subtype of acute leukemia, a cancer of the white blood cells. ALL is a malignant disease of bone marrow and the most common malignancy diagnosed in children. The malignant cells are lymphoid precursor cells (lymphoblasts) that are arrested in an early stage of development. The lymphoblasts replace the normal marrow elements, resulting in a marked decrease in the production of normal blood cells. Consequently, anemia, thrombocytopenia, and neutropenia occur to varying degrees. The lymphoblasts also proliferate in organs other than the marrow, particularly the liver, spleen, and lymphonodes. Belongs to the ARID5B family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 10q21.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; transcription coactivator activity

Biological Process: fat cell differentiation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; adrenal gland development; multicellular organism growth; male gonad development; platelet-derived growth factor receptor signaling pathway; palate development; negative regulation of transcription from RNA polymerase II promoter; liver development; post-embryonic development; regulation of transcription from RNA polymerase II promoter; skeletal morphogenesis; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; kidney development; cell development; muscle morphogenesis; female gonad development