Rabbit anti-Human ARID5B Polyclonal Antibody | anti-ARID5B antibody

ARID5B, ID (ARID5B, DESRT, MRF2, AT-rich interactive domain-containing protein 5B, MRF1-like protein, Modulator recognition factor 2) (AP)

Cat. Num. AAA6275061

• Gene Names: ARID5B; MRF2; DESRT; MRF-2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ARID5B; Polyclonal Antibody; ID (ARID5B; DESRT; MRF2; AT-rich interactive domain-containing protein 5B; MRF1-like protein; Modulator recognition factor 2) (AP); Modulator recognition factor 2; anti-ARID5B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 1188

• Applicable Applications for anti-ARID5B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ARID5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 388-418 amino acids from the Central region of human ARID5B.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ARID5B Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ARID5B antibody detected the ARID5B protein (arrow).)

Related Product Information for anti-ARID5B antibody

DNA-binding protein that binds to the 5'-AATA[CT]-3' core sequence. Probably acts as a transcription regulator. Represses the cytomegalovirus enhancer. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may act as a regulator of smooth muscle cell differentiation and proliferation. May be involved in lipid stores.

Product Categories/Family for anti-ARID5B antibody

Antibodies; Transcription Factors

References

Healy, J., et al. Haematologica 95(9):1608-1611(2010). Yang, W., et al. Leukemia 24(4):894-896(2010). Prasad, R.B., et al. Blood 115(9):1765-1767(2010). Papaemmanuil, E., et al. Nat. Genet. 41(9):1006-1010(2009). Trevino, L.R., et al. Nat. Genet. 41(9):1001-1005(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 74136549
• NCBI GeneID: 84159
• NCBI Accession #: NP_115575.1
• NCBI GenBank Nucleotide #: NP_115575.1
• UniProt Accession #: Q14865
• NCBI Official Full Name: AT-rich interactive domain-containing protein 5B isoform 1
• NCBI Official Synonym Full Names: AT-rich interaction domain 5B
• NCBI Official Symbol: ARID5B
• NCBI Official Synonym Symbols: MRF2; DESRT; MRF-2
• NCBI Protein Information: AT-rich interactive domain-containing protein 5B
• UniProt Protein Name: AT-rich interactive domain-containing protein 5B
• Protein Family: AT-rich interactive domain-containing protein
• UniProt Gene Name: ARID5B
• UniProt Synonym Gene Names: DESRT; MRF2; ARID domain-containing protein 5B; MRF-2
• UniProt Entry Name: ARI5B_HUMAN

NCBI Description

This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

Mrf-2: Transcription coactivator that binds to the 5'-AATA[CT]- 3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2- ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. Defects in ARID5B may be a cause of susceptibility to coronary atherosclerosis in the Japanese population. Defects in ARID5B may be a cause of susceptibility to acute lymphoblastic leukemia (ALL). ALL is a subtype of acute leukemia, a cancer of the white blood cells. ALL is a malignant disease of bone marrow and the most common malignancy diagnosed in children. The malignant cells are lymphoid precursor cells (lymphoblasts) that are arrested in an early stage of development. The lymphoblasts replace the normal marrow elements, resulting in a marked decrease in the production of normal blood cells. Consequently, anemia, thrombocytopenia, and neutropenia occur to varying degrees. The lymphoblasts also proliferate in organs other than the marrow, particularly the liver, spleen, and lymphonodes. Belongs to the ARID5B family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 10q21.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; transcription coactivator activity

Biological Process: fat cell differentiation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; adrenal gland development; multicellular organism growth; male gonad development; platelet-derived growth factor receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; palate development; liver development; post-embryonic development; regulation of transcription from RNA polymerase II promoter; skeletal morphogenesis; positive regulation of transcription factor activity; kidney development; negative regulation of transcription, DNA-dependent; cell development; muscle morphogenesis; female gonad development