Human AT rich interactive domain containing protein 5B (ARID5B) ELISA Kit | ARID5B elisa kit

Human AT rich interactive domain containing protein 5B (ARID5B) ELISA Kit

Cat. Num. AAA7212187

• Gene Names: ARID5B; MRF2; DESRT; MRF-2
• Reactivity: Human
• Synonyms: AT rich interactive domain containing protein 5B (ARID5B); Human AT rich interactive domain containing protein 5B (ARID5B) ELISA Kit; ARID5B elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This ARID5B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARID5B. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ARID5B elisa kit

Principle of the assay: ARID5B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARID5B antibody and an ARID5B-HRP conjugate. The assay sample and buffer are incubated together with ARID5B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARID5B concentration since ARID5B from samples and ARID5B-HRP conjugate compete for the anti-ARID5B antibody binding site. Since the number of sites is limited, as more sites are occupied by ARID5B from the sample, fewer sites are left to bind ARID5B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARID5B concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ARID5B elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 209572763
• NCBI GeneID: 84159
• Molecular Weight: 36,282 Da
• NCBI Official Full Name: AT-rich interactive domain-containing protein 5B
• NCBI Official Synonym Full Names: AT rich interactive domain 5B (MRF1-like)
• NCBI Official Symbol: ARID5B
• NCBI Official Synonym Symbols: MRF2; DESRT; MRF-2
• NCBI Protein Information: AT-rich interactive domain-containing protein 5B; MRF1-like protein; ARID domain-containing protein 5B; modulator recognition factor 2 (MRF2)
• UniProt Protein Name: AT-rich interactive domain-containing protein 5B
• Protein Family: AT-rich interactive domain-containing protein
• UniProt Gene Name: ARID5B
• UniProt Synonym Gene Names: DESRT; MRF2; ARID domain-containing protein 5B; MRF-2
• UniProt Entry Name: ARI5B_HUMAN

NCBI Description

This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

Mrf-2: Transcription coactivator that binds to the 5'-AATA[CT]- 3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2- ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. Defects in ARID5B may be a cause of susceptibility to coronary atherosclerosis in the Japanese population. Defects in ARID5B may be a cause of susceptibility to acute lymphoblastic leukemia (ALL). ALL is a subtype of acute leukemia, a cancer of the white blood cells. ALL is a malignant disease of bone marrow and the most common malignancy diagnosed in children. The malignant cells are lymphoid precursor cells (lymphoblasts) that are arrested in an early stage of development. The lymphoblasts replace the normal marrow elements, resulting in a marked decrease in the production of normal blood cells. Consequently, anemia, thrombocytopenia, and neutropenia occur to varying degrees. The lymphoblasts also proliferate in organs other than the marrow, particularly the liver, spleen, and lymphonodes. Belongs to the ARID5B family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 10q21.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; transcription coactivator activity

Biological Process: fat cell differentiation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; adrenal gland development; multicellular organism growth; male gonad development; platelet-derived growth factor receptor signaling pathway; palate development; negative regulation of transcription from RNA polymerase II promoter; liver development; post-embryonic development; regulation of transcription from RNA polymerase II promoter; skeletal morphogenesis; positive regulation of transcription factor activity; kidney development; negative regulation of transcription, DNA-dependent; cell development; muscle morphogenesis; female gonad development