APEX2 sirna

APEX2 siRNA (Human)

Cat. Num. AAA8209169

• Gene Names: APEX2; APE2; XTH2; ZGRF2; APEXL2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APEX2; APEX2 siRNA (Human); APE2; APEXL2; XTH2; DNA-(apurinic or apyrimidinic site) lyase 2; AP endonuclease XTH2; APEX nuclease 2; APEX nuclease-like 2; Apurinic-apyrimidinic endonuclease 2; AP endonuclease 2; APEX2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: APEX2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 518

• Applicable Applications for APEX2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human APEX2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APEX2 sirna

siRNA to inhibit APEX2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 18375507
• NCBI GeneID: 27301
• NCBI Accession #: NP_055296.2
• NCBI GenBank Nucleotide #: NM_014481.3
• UniProt Accession #: Q9UBZ4; Q9Y5X7
• Molecular Weight: 57,401 Da
• NCBI Official Full Name: DNA-(apurinic or apyrimidinic site) lyase 2 isoform 1
• NCBI Official Synonym Full Names: APEX nuclease (apurinic/apyrimidinic endonuclease) 2
• NCBI Official Symbol: APEX2
• NCBI Official Synonym Symbols: APE2; XTH2; ZGRF2; APEXL2
• NCBI Protein Information: DNA-(apurinic or apyrimidinic site) lyase 2
• UniProt Protein Name: DNA-(apurinic or apyrimidinic site) lyase 2
• Protein Family: DNA-(apurinic or apyrimidinic site) lyase
• UniProt Gene Name: APEX2
• UniProt Synonym Gene Names: APE2; APEXL2; XTH2; AP endonuclease 2
• UniProt Entry Name: APEX2_HUMAN

NCBI Description

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

APEX2: Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. Belongs to the DNA repair enzymes AP/ExoA family.

Protein type: Mitochondrial; Lyase; EC 4.2.99.18

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: intracellular membrane-bound organelle; mitochondrial inner membrane; nucleolus; nucleus

Molecular Function: DNA-(apurinic or apyrimidinic site) lyase activity; DNA binding; zinc ion binding; double-stranded DNA specific 3'-5' exodeoxyribonuclease activity

Biological Process: base-excision repair; DNA catabolic process, exonucleolytic; cell cycle; DNA catabolic process, endonucleolytic; DNA recombination