Test Data
APEX2 cdna clone
APEX2 cDNA Clone
Test Data
Test Data
NCBI and Uniprot Product Information
NCBI Description
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]
Uniprot Description
APEX2: Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. Belongs to the DNA repair enzymes AP/ExoA family.
Protein type: EC 4.2.99.18; Lyase; Mitochondrial
Chromosomal Location of Human Ortholog: Xp11.21
Cellular Component: intracellular membrane-bound organelle; nucleolus; nucleus
Molecular Function: DNA-(apurinic or apyrimidinic site) lyase activity; double-stranded DNA specific 3'-5' exodeoxyribonuclease activity
Biological Process: base-excision repair
Research Articles on APEX2
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Product Notes
The APEX2 apex2 (Catalog #AAA1266384) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: ATGTTGCGCG TGGTGAGCTG GAACATCAAT GGGATTCGGA GACCCCTGCA AGGGGTGGCA AATCAGGAAC CCAGCAACTG TGCCGCCGTG GCCGTGGGGC GCATTTTGGA CGAGCTGGAT GCGGATATCG TCTGTCTCCA GGAAACCAAA GTGACCAGGG ATGCACTGAC AGAGCCCCTG GCTATCGTTG AGGGTTATAA CTCCTATTTC AGCTTCAGCC GCAACCGTAG CGGCTATTCT GGTGTAGCCA CCTTCTGTAA GGACAATGCT ACCCCAGTGG CTGCTGAAGA AGGCCTGAGT GGCCTGTTTG CCACCCAGAA TGGGGATGTT GGTTGCTATG GAAACATGGA TGAGTTTACC CAAGAGGAAC TCCGGGCTCT GGATAGTGAG GGCAGGGCCC TCCTCACACA GCATAAGATC CGCACATGGG AAGGTAAGGA GAAGACCTTG ACCCTAATCA ACGTGTACTG CCCCCATGCG GACCCTGGGA GGCCTGAGCG GCTAGTCTTT AAGATGCGCT TCTATCGTTT GCTGCAAATC CGAGCAGAAG CCCTCCTGGC GGCAGGCAGC CATGTGATCA TTCTGGGTGA CCTGAATACA GCCCACCGCC CCATTGACCA CTGGGATGCA GTCAACCTGG AATGCTTTGA AGAGGACCCA GGGCGCAAGT GGATGGACAG CTTGCTCAGT AACTTGGGGT GCCAGTCTGC CTCTCATGTA GGGCCCTTCA TCGATAGCTA CCGCTGCTTC CAACCAAAGC AGGAGGGGGC CTTCACCTGC TGGTCAGCAG TCACTGGCGC CCGCCATCTC AACTATGGCT CCCGGCTTGA CTATGTGCTG GGGGACAGGA CCCTGGTCAT AGACACCTTT CAGGCCTCTT TCCTGCTGCC TGAGGTGATG GGCTCTGACC ACTGCCCTGT GGGTGCAGTC TTGAGTGTGT CCTCTGTGCC TGCAAAACAG TGCCCACCTC TGTGCACCCG CTTCCTCCCT GAGTTTGCAG GCACCCAGCT CAAGATCCTT CGCTTCCTAG TTCCTCTCGA ACAAAGTCCT GTGTTGGAGC AGTCGACGCT GCAGCACAAC AATCAAACCC GGGTACAGAC ATGCCAAAAC AAAGCCCAAG TGCGCTCAAC CAGGCCTCAG CCCAGTCAGG TTGGCTCTAG CAGAGGCCAG AAAAACCTGA AGAGCTACTT TCAGCCCTCC CCTAGCTGTC CCCAAGCCTC TCCTGACATA GAGCTGCCTA GCCTACCACT GATGAGCGCC CTCATGACCC CGAAGACTCC AGAAGAGAAG GCAGTGGCCA AAGTGGTGAA GGGGCAGGCC AAGACTTCAG AAGCCAAAGA TGAGAAGGAG TTACGGACCT CATTCTGGAA GTCTGTGCTG GCGGGGCCCT TGCGCACACC CCTCTGTGGG GGCCACAGGG AGCCATGTGT GATGCGTACT GTGAAGAAGC CAGGACCCAA CTTGGGCCGC CGCTTCTACA TGTGTGCCAG GCCCCGGGGT CCTCCCACTG ACCCCTCCTC CCGGTGCAAC TTCTTCCTCT GGAGCAGGCC CAGCTGA. It is sometimes possible for the material contained within the vial of "APEX2, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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