Rabbit anti-Human APEX2 Polyclonal Antibody | anti-APEX2 antibody

APEX2 (AP Endonuclease XTH2, APEX Nuclease-like 2, APEX Nuclease 2, Apurinic-apyrimidinic Endonuclease 2, AP Endonuclease 2, DNA-(apurinic or apyrimidinic site) Lyase 2, APE2, APEXL2, XTH2) (FITC)

Cat. Num. AAA6463415

• Gene Names: APEX2; APE2; XTH2; ZGRF2; APEXL2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: APEX2; Polyclonal Antibody; APEX2 (AP Endonuclease XTH2; APEX Nuclease-like 2; APEX Nuclease 2; Apurinic-apyrimidinic Endonuclease 2; AP Endonuclease 2; DNA-(apurinic or apyrimidinic site) Lyase 2; APE2; APEXL2; XTH2) (FITC); anti-APEX2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human APEX2.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-APEX2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide (KLH conjugated) located between a portion within aa150-179 in the center region of human APEX2
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of APEX2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). APEX2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with APEX2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(APEX2 Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-APEX2 antibody

Antibodies; Enzymes

References

1. Hadi, M.Z., et.al., J. Mol. Biol. 316(3), 853-866 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 18375507
• NCBI GeneID: 27301
• NCBI Accession #: NP_055296.2
• NCBI GenBank Nucleotide #: NM_014481.3
• UniProt Accession #: Q9UBZ4; Q9Y5X7
• Molecular Weight: 57,401 Da
• NCBI Official Full Name: DNA-(apurinic or apyrimidinic site) lyase 2 isoform 1
• NCBI Official Synonym Full Names: apurinic/apyrimidinic endodeoxyribonuclease 2
• NCBI Official Symbol: APEX2
• NCBI Official Synonym Symbols: APE2; XTH2; ZGRF2; APEXL2
• NCBI Protein Information: DNA-(apurinic or apyrimidinic site) lyase 2
• UniProt Protein Name: DNA-(apurinic or apyrimidinic site) lyase 2
• Protein Family: DNA-(apurinic or apyrimidinic site) lyase
• UniProt Gene Name: APEX2
• UniProt Synonym Gene Names: APE2; APEXL2; XTH2; AP endonuclease 2
• UniProt Entry Name: APEX2_HUMAN

NCBI Description

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

APEX2: Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. Belongs to the DNA repair enzymes AP/ExoA family.

Protein type: Lyase; EC 4.2.99.18; Mitochondrial

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: intracellular membrane-bound organelle; mitochondrial inner membrane; nucleolus; nucleus

Molecular Function: DNA-(apurinic or apyrimidinic site) lyase activity; DNA binding; zinc ion binding; double-stranded DNA specific 3'-5' exodeoxyribonuclease activity

Biological Process: base-excision repair; DNA catabolic process, exonucleolytic; cell cycle; DNA catabolic process, endonucleolytic; DNA recombination