ADAM7 sirna

ADAM7 siRNA (Human)

Cat. Num. AAA8224084

• Gene Names: ADAM7; EAPI; GP83; GP-83; ADAM 7; ADAM-7
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADAM7; ADAM7 siRNA (Human); GP83; Disintegrin and metalloproteinase domain-containing protein 7; ADAM 7; Sperm maturation-related glycoprotein GP-83; ADAM7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADAM7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 754

• Applicable Applications for ADAM7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADAM7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADAM7 sirna

siRNA to inhibit ADAM7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 114326453
• NCBI GeneID: 8756
• NCBI Accession #: NP_003808.2
• NCBI GenBank Nucleotide #: NM_003817.3
• UniProt Accession #: Q9H2U9; O75959; Q6PEJ6; A8K8X7
• Molecular Weight: 24,414 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 7 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 7
• NCBI Official Symbol: ADAM7
• NCBI Official Synonym Symbols: EAPI; GP83; GP-83; ADAM 7; ADAM-7
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 7
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 7
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM7
• UniProt Synonym Gene Names: GP83; ADAM 7
• UniProt Entry Name: ADAM7_HUMAN

NCBI Description

This gene encodes a member of the ADAMs family of zinc proteases. These transmembrane proteins play roles in multiple processes including cell signaling, adhesion and migration. The encoded protein lacks protease activity and may play roles in protein-protein interactions and cell adhesion processes including sperm-egg fusion. Mutations in this gene may be involved in the progression of melanoma. [provided by RefSeq, Oct 2011]

Uniprot Description

ADAM7: May play an important role in male reproduction including sperm maturation and gonadotrope function. This is a non catalytic metalloprotease-like protein. Has been found to be frequently mutated in melanoma. ADAM7 mutations may play a role in melanoma progression and metastasis.

Protein type: Protease; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: plasma membrane; integral to membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: proteolysis