IDH2 blocking peptide

IDH2 Blocking Peptide

Cat. Num. AAA8218339

• Gene Names: IDH2; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: IDH2; IDH2 Blocking Peptide; Isocitrate dehydrogenase [NADP]; mitochondrial; IDH; ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; IDH2 blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Rat, Monkey
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 400

• Applicable Applications for IDH2 blocking peptide: Blocking (BL)
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Directions for Use: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IDH2 blocking peptide

The peptide is used to block Anti-IDH2 Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 583966148
• NCBI GeneID: 3418
• NCBI Accession #: NP_001276839.1
• NCBI GenBank Nucleotide #: NM_001289910.1
• UniProt Accession #: P48735; Q96GT3; B2R6L6; B4DFL2
• Molecular Weight: 45,180 Da
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 2 (NADP+), mitochondrial
• NCBI Official Symbol: IDH2
• NCBI Official Synonym Symbols: IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• NCBI Protein Information: isocitrate dehydrogenase [NADP], mitochondrial
• UniProt Protein Name: Isocitrate dehydrogenase [NADP], mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH2
• UniProt Synonym Gene Names: IDH
• UniProt Entry Name: IDHP_HUMAN

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

IDH2: a mitochondrial oxidoreductase that catalyzes the third step of the citric acid cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Can also use oxalosuccinate as a substrate. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH2 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. May tightly associate or interact with the pyruvate dehydrogenase complex. A somatic mutation resulting in the R172K conversion results in a neomorphic enzyme activity that converts isocitrate to R(-)-2-hydroxyglutarate (2HG) occurs in a significant percentage of patients with cytogenetically normal acute myeloid leukemia (AML). Elevated levels of 2HG are also correlated with an elevated risk of malignant brain tumors.

Protein type: Oxidoreductase; EC 1.1.1.42; Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: magnesium ion binding; isocitrate dehydrogenase (NADP+) activity; NAD binding

Biological Process: glyoxylate cycle; isocitrate metabolic process; cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; 2-oxoglutarate metabolic process

Disease: D-2-hydroxyglutaric Aciduria 2