Human Isocitrate dehydrogenase [NADP], mitochondrial ELISA Kit | IDH2 elisa kit

Human Isocitrate dehydrogenase [NADP], mitochondrial ELISA Kit

Cat. Num. AAA288993

• Gene Names: IDH2; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• Reactivity: Human
• Synonyms: Isocitrate dehydrogenase [NADP]; mitochondrial; Human Isocitrate dehydrogenase [NADP]; mitochondrial ELISA Kit; IDH; ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; IDH2; 1.1.1.42; IDH2 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Isocitrate dehydrogenase [NADP], mitochondrial,IDH
• Sequence Length: 400

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.312-20 ng/mL
• Sensitivity: < 0.098 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.4%
• Inter-assay Precision: Inter-Assay CV: <=7.1%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IDH2 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 583966148
• NCBI GeneID: 3418
• NCBI Accession #: NP_001276839.1
• NCBI GenBank Nucleotide #: NM_001289910.1
• UniProt Accession #: P48735; Q96GT3; B2R6L6; B4DFL2
• Molecular Weight: 45,180 Da
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 2 (NADP+), mitochondrial
• NCBI Official Symbol: IDH2
• NCBI Official Synonym Symbols: IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• NCBI Protein Information: isocitrate dehydrogenase [NADP], mitochondrial
• UniProt Protein Name: Isocitrate dehydrogenase [NADP], mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH2
• UniProt Synonym Gene Names: IDH
• UniProt Entry Name: IDHP_HUMAN

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

IDH2: a mitochondrial oxidoreductase that catalyzes the third step of the citric acid cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Can also use oxalosuccinate as a substrate. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH2 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. May tightly associate or interact with the pyruvate dehydrogenase complex. A somatic mutation resulting in the R172K conversion results in a neomorphic enzyme activity that converts isocitrate to R(-)-2-hydroxyglutarate (2HG) occurs in a significant percentage of patients with cytogenetically normal acute myeloid leukemia (AML). Elevated levels of 2HG are also correlated with an elevated risk of malignant brain tumors.

Protein type: Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; Other Amino Acids Metabolism - glutathione; EC 1.1.1.42

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial matrix; mitochondrion; peroxisome

Molecular Function: isocitrate dehydrogenase (NADP+) activity; magnesium ion binding; NAD binding

Biological Process: 2-oxoglutarate metabolic process; carbohydrate metabolic process; cellular metabolic process; glyoxylate cycle; isocitrate metabolic process; tricarboxylic acid cycle

Disease: D-2-hydroxyglutaric Aciduria 2