Rabbit IDH2 Polyclonal Antibody | anti-IDH2 antibody

IDH2 Antibody

Cat. Num. AAA9411113

• Gene Names: IDH2; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: IDH2; Polyclonal Antibody; IDH2 Antibody; Mitochondrial NADP-isocitrate dehydrogenase; IDH; IDP; IDHM; IDPM; ICD-M; mNADP-IDH; anti-IDH2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 452

• Applicable Applications for anti-IDH2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 17 amino acid peptide near the amino terminus of human IDH2.
• Target Name: IDH2
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of IDH2 in human heart tissue lysate with IDH2 antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of IDH2 in mouse heart tissue with IDH2 antibody at 5 ug/mL.)

Related Product Information for anti-IDH2 antibody

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Two NADP(+)-dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial. Both IDH1 and IDH2 play significant roles in the tricarboxylic acid cycle (TCA), and defects in IDH1 as well as IDH2 have been implicated in the development of glioma as well as other malignancies.

Product Categories/Family for anti-IDH2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 28178832
• NCBI GeneID: 3418
• NCBI Accession #: NP_002159
• NCBI GenBank Nucleotide #: NM_002168.3
• Molecular Weight: 45,180 Da
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 2 (NADP+), mitochondrial
• NCBI Official Symbol: IDH2
• NCBI Official Synonym Symbols: IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• NCBI Protein Information: isocitrate dehydrogenase [NADP], mitochondrial
• UniProt Protein Name: Isocitrate dehydrogenase [NADP], mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH2
• UniProt Synonym Gene Names: IDH
• UniProt Entry Name: IDHP_HUMAN

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

IDH2: a mitochondrial oxidoreductase that catalyzes the third step of the citric acid cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Can also use oxalosuccinate as a substrate. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH2 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. May tightly associate or interact with the pyruvate dehydrogenase complex. A somatic mutation resulting in the R172K conversion results in a neomorphic enzyme activity that converts isocitrate to R(-)-2-hydroxyglutarate (2HG) occurs in a significant percentage of patients with cytogenetically normal acute myeloid leukemia (AML). Elevated levels of 2HG are also correlated with an elevated risk of malignant brain tumors.

Protein type: Mitochondrial; EC 1.1.1.42; Other Amino Acids Metabolism - glutathione; Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: magnesium ion binding; isocitrate dehydrogenase (NADP+) activity; NAD binding

Biological Process: isocitrate metabolic process; glyoxylate cycle; cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; 2-oxoglutarate metabolic process

Disease: D-2-hydroxyglutaric Aciduria 2