SUV420H2 Recombinant Protein | KMT5C recombinant protein

SUV420H2 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8121033

• Gene Names: SUV420H2; KMT5C
• Purity: >80% as determined by SDS-PAGE
• Synonyms: SUV420H2; SUV420H2 Protein; Human; Recombinant (His & GST Tag); Human SUV420H2 Protein (His & GST Tag); KMT5C Protein; suppressor of variegation 4-20 homolog 2 (Drosophila); KMT5C recombinant protein

• Host: E Coli
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 0.5M NaCl, 30% Glycerol, 0.05% Teween, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly2-Leu280

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the amino acid sequence (Gly 2-Leu 280) of human SUV420H2 (NP_116090.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for KMT5C recombinant protein

Background: Histone-lysine N-methyltransferase SUV42H2, also known as Suppressor of variegation 4-2 homolog 2, Su(var)4-2 homolog 2, Lysine N-methyltransferase 5C, SUV42H2 and KMT5C, is nucleus protein that belongs to the histone-lysine methyltransferase family and Suvar4-2 subfamily. SUV42H2 is a histone methyltransferase that specifically trimethylates 'Lys-2' of histone H4. H4 'Lys-2' trimethylation represents a specific tag for epigenetic transcriptional repression. SUV42H2 mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV42H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). FRAP experiments reveal that SUV42H2 is strongly associated with pericentric heterochromatin. The fraction of SUV42H2 captured and characterized by TAP/MS is a soluble fraction which may be in a stable association with HP1. SUV42H2 may be recruited to heterochromatin in association with HP1, and stably maintained at its heterochromatin sites in an HP1-independent fashion.

References

Wan D. et al.,2004, Proc. Natl. Acad. Sci. USA. 101:15724-9. Souza, PP. et al., 2009, BMC Cell Biol. 10: 41. Matsui,T. et al., 2010, Nature. 464 (7290): 927-31.

NCBI and Uniprot Product Information

• NCBI GI #: 31543169
• NCBI GeneID: 84787
• NCBI Accession #: NP_116090.2
• NCBI GenBank Nucleotide #: NM_032701.3
• UniProt Accession #: Q86Y97; Q8WZ10; Q9BRZ6
• Molecular Weight: 52,113 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SUV420H2
• NCBI Official Synonym Full Names: suppressor of variegation 4-20 homolog 2 (Drosophila)
• NCBI Official Symbol: SUV420H2
• NCBI Official Synonym Symbols: KMT5C
• NCBI Protein Information: histone-lysine N-methyltransferase SUV420H2; suv4-20h2; su(var)4-20 homolog 2; lysine N-methyltransferase 5C
• UniProt Protein Name: Histone-lysine N-methyltransferase SUV420H2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SUV420H2
• UniProt Synonym Gene Names: KMT5C
• UniProt Entry Name: SV422_HUMAN

Uniprot Description

SUV420H2: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: nucleoplasm; centric heterochromatin; nuclear heterochromatin; condensed nuclear chromosome, pericentric region

Molecular Function: protein binding; histone lysine N-methyltransferase activity (H4-K20 specific)

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent