Streptavidin Protein
Streptavidin (Cy5)
Applications
Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified
Synonyms
Streptavidin; Streptavidin (Cy5); Streptavidin protein
Host
Streptomyces avidinii
Purity/Purification
Purified
Form/Format
Supplied as a liquid in 0.1% sodium azide. Labeled with CY5.
Applicable Applications for Streptavidin protein
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry and Immunocytochemistry.
Dilution: Immunofluorescence: 1:20-1:50
Flow Cytometry: 1:50-1:200
Dilution: Immunofluorescence: 1:20-1:50
Flow Cytometry: 1:50-1:200
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 6 months at 4 degree C. Freezing CY5 conjugates will result in a substantial loss of activity. CY5 conjugates are sensitive to light.
Related Product Information for Streptavidin protein
Streptavidin (MW ~66kD) binds specifically with biotin (244D). It is derived from the bacterium Streptomyces avidinii and bears a remarkable similarity to chicken egg-white avidin both in three-dimensional structure and its ability to bind biotin with extremely high affinity (Kd=10e-15M). It is a tetrameric protein capable of binding up to 4 biotin molecules. Unlike avidin, Streptavidin is non-glycosylated and is essentially neutral in charge, whereas avidin (pH 10.5) is basic at neutral pH. Because of this, streptavidin has considerably less non-specific binding resulting in less background. It has replaced avidin as the reagent of choice for most applications where protein interactions may cause background.
Product Categories/Family for Streptavidin protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Streptavidin (Catalog #AAA634373) is a Protein produced from Streptomyces avidinii and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Streptavidin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Dilution: Immunofluorescence: 1:20-1:50 Flow Cytometry: 1:50-1:200. Researchers should empirically determine the suitability of the Streptavidin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Streptavidin, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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