Western Blot
(Western Blot analysis using Streptavidin antibodyStreptavidin and Biotin conj ugated Rabbit anti-Trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Donkey anti Rabbit (Dylight 649) (1:10, 000 1. 5 hr RT in blocking buffer) and imaged on the BioRad VersaDoc System. )
Streptavidin Antibody
Streptavidin antibody (FITC)
Reactivity
Does not cross-react with Avid. Specific for Streptavidin
Applications
Flow Cytometry, Immunofluorescence
Purity
Streptavidin antibody (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Synonyms
Streptavidin; Streptavidin antibody (FITC); Polyclonal Streptavidin; Anti-Streptavidin; anti-Streptavidin antibody
Reactivity
Does not cross-react with Avid. Specific for Streptavidin
Specificity
Streptomyces avidinii streptavidin
Purity/Purification
Streptavidin antibody (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Form/Format
IgG fraction supplied as a liquid in 20mM Potassium Phosphate, 150mM Sodium Chloride, pH 7.2, with 10mg/ml BSA (IgG and Protease free) as a stabilizer, and 0.01% Sodium Azide as a preservative.
Concentration
5.0mg/ml (by UV absorbance at 280nm) (varies by lot)
Applicable Applications for anti-Streptavidin antibody
Flow Cytometry (FC), Immunofluorescence (IF)
Application Notes
FC: 1:2,000-1:10,000
IF: 1:500-1:2,500
OTHER: FC: 1:2,000-1:10,000
IF: 1:500-1:2,500
OTHER: FC: 1:2,000-1:10,000
Immunogen
Streptavidin (Streptomyces avidinii)
F/P Ratio
4.0 moles FITC per mole of Rabbit IgG
Tag
FITC
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Biological Significance
Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin (also known as vitamin B7); the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of ˜10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants, detergents and proteolytic enzymes.
Preparation and Storage
Short Term: 4°C
Long Term: -20°C
Long Term: -20°C
Western Blot
(Western Blot analysis using Streptavidin antibodyStreptavidin and Biotin conj ugated Rabbit anti-Trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Donkey anti Rabbit (Dylight 649) (1:10, 000 1. 5 hr RT in blocking buffer) and imaged on the BioRad VersaDoc System. )
Western Blot
(Western Blot analysis using Streptavidin antibodyStreptavidin and Biotin conj ugated Rabbit anti-Trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Donkey anti Rabbit (Dylight 649) (1:10, 000 1. 5 hr RT in blocking buffer) and imaged on the BioRad VersaDoc System. )
Related Product Information for anti-Streptavidin antibody
Rabbit polyclonal Streptavidin antibody (FITC) conjugated
Product Categories/Family for anti-Streptavidin antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Streptavidin (Catalog #AAA535116) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Streptavidin antibody (FITC) reacts with Does not cross-react with Avid. Specific for Streptavidin and may cross-react with other species as described in the data sheet. AAA Biotech's Streptavidin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunofluorescence (IF). FC: 1:2,000-1:10,000 IF: 1:500-1:2,500 OTHER: FC: 1:2,000-1:10,000. Researchers should empirically determine the suitability of the Streptavidin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Streptavidin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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