Testing Data
(Sequence)
Steroid 5 Alpha Reductase 2 (SRD5a2) Recombinant Protein | SRD5a2 recombinant protein
Recombinant Steroid 5 Alpha Reductase 2 (SRD5a2)
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Steroid 5 Alpha Reductase 2 (SRD5a2); Recombinant Steroid 5 Alpha Reductase 2 (SRD5a2); 3-Oxo-5 Alpha-Steroid Delta 4-Dehydrogenase Alpha 2; SRD5a2 recombinant protein
Host
E.coli
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM DTT, 0.01% SKL, 5% Trehalose .
Concentration
Original Concentration: 200ug/mL (varies by lot)
Applicable Applications for SRD5a2 recombinant protein
Positive Control; Immunogen; SDS-PAGE (SDS); Western Blot (WB)
Application Notes
May be suitable for use in other assays to be determined by the end user.
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
His90~Phe254
Tags
N-terminal His and GST Tag
Subcellular Location
Membrane
Traits
Freeze-dried powder
Predicted isoelectric point
9.3
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Testing Data
(Sequence)
Testing Data
(Sequence)
Testing Data
(Identification)
Testing Data
(Identification)
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted Molecular Mass: 49.2kDa
Accurate Molecular Mass: 47kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 47kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
steroid 5-alpha-reductase 2
NCBI Official Synonym Full Names
steroid 5 alpha-reductase 2
NCBI Official Symbol
SRD5A2
NCBI Protein Information
3-oxo-5-alpha-steroid 4-dehydrogenase 2
Protein Family
NCBI Description
This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]
Research Articles on SRD5a2
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Product Notes
The SRD5a2 (Catalog #AAA2105637) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Steroid 5 Alpha Reductase 2 (SRD5a2) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE (SDS); Western Blot (WB). May be suitable for use in other assays to be determined by the end user. Researchers should empirically determine the suitability of the SRD5a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Steroid 5 Alpha Reductase 2 (SRD5a2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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