Western Blot (WB)
(Western Blot (WB) analysis of PGLS (A226) pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug))
Rabbit anti-Human PGLS Polyclonal Antibody | anti-PGLS antibody
PGLS Polyclonal Antibody
Gene Names
PGLS; 6PGL; HEL-S-304
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
PGLS; Polyclonal Antibody; PGLS Polyclonal Antibody; 6-phosphogluconolactonase; 6PGL; anti-PGLS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
PGLS polyclonal antibody detects endogenous levels of PGLS protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
258
Applicable Applications for anti-PGLS antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 213-258 of Human PGLS.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of PGLS (A226) pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of PGLS (A226) pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug))
Related Product Information for anti-PGLS antibody
PGLS (6-phosphogluconolactonase), also known as 6PGL, is a 258 amino acid protein that belongs to the glucosamine/galactosamine-6-phosphate isomerase family and the 6-phosphogluconolactonase subfamily. Localizing to cytoplasm, PGLS is a particularly active enzyme that catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate, which is the second step of the pentose phosphate pathway. Highly conserved, PGLS shares 33% to 37% sequence similarity with yeast Sol1, Sol2, Sol3 and Sol4, 26% similarity with the C-terminal portion of human H6PD, 20% to 25% similarity with bacterial devB proteins and 17% similarity with human GNPDA1. PGLS erythrocyte deficiency, an autosomal dominant disorder, in conjunction with G6PD deficiency, may play a role in hemolytic anemia.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 28kDa
NCBI Official Full Name
6-phosphogluconolactonase
NCBI Official Synonym Full Names
6-phosphogluconolactonase
NCBI Official Symbol
PGLS
NCBI Official Synonym Symbols
6PGL; HEL-S-304
NCBI Protein Information
6-phosphogluconolactonase
UniProt Protein Name
6-phosphogluconolactonase
UniProt Gene Name
PGLS
UniProt Synonym Gene Names
6PGL
UniProt Entry Name
6PGL_HUMAN
Uniprot Description
PGLS: Hydrolysis of 6-phosphogluconolactone to 6- phosphogluconate. Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.
Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Hydrolase; EC 3.1.1.31
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: cytosol
Molecular Function: monosaccharide binding; 6-phosphogluconolactonase activity
Biological Process: pentose-phosphate shunt; carbohydrate metabolic process; pentose-phosphate shunt, oxidative branch; pathogenesis
Similar Products
Product Notes
The PGLS pgls (Catalog #AAA3006079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGLS Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PGLS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PGLS pgls for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGLS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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