Mouse Signal Transducer And Activator Of Transcription 6 ELISA Kit | STAT6 elisa kit

Mouse Signal Transducer And Activator Of Transcription 6 ELISA Kit

Cat. Num. AAA725637

• Gene Names: STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
• Reactivity: Mouse
• Synonyms: Signal Transducer And Activator Of Transcription 6; Mouse Signal Transducer And Activator Of Transcription 6 ELISA Kit; STAT6 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of STAT6. No significant cross-reactivity or interference between STAT6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between STAT6 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STAT6 elisa kit

Intended Uses: This STAT6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of MouseSTAT6. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: STAT6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-STAT6 antibody and an STAT6-HRP conjugate. The assay sample and buffer are incubated together with STAT6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STAT6 concentration since STAT6 from samples and STAT6-HRP conjugate compete for the anti-STAT6 antibody binding site. Since the number of sites is limited, as more sites are occupied by STAT6 from the sample, fewer sites are left to bind STAT6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STAT6 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for STAT6 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 23397678
• NCBI GeneID: 6778
• NCBI Accession #: NP_003144.3
• NCBI GenBank Nucleotide #: NM_003153.4
• Molecular Weight: 81,748 Da
• NCBI Official Full Name: signal transducer and activator of transcription 6 isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 6, interleukin-4 induced
• NCBI Official Symbol: STAT6
• NCBI Official Synonym Symbols: STAT6B; STAT6C; D12S1644; IL-4-STAT
• NCBI Protein Information: signal transducer and activator of transcription 6; STAT, interleukin4-induced; transcription factor IL-4 STAT
• UniProt Protein Name: Signal transducer and activator of transcription 6
• Protein Family: Signal transducer and transcription activator
• UniProt Gene Name: STAT6
• UniProt Entry Name: STAT6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

STAT6: transcription factor of the STAT family. Plays a central role in IL4-mediated biological responses. Induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. May function in the differentiation of T helper 2 cells and class switch of immunoglobulins. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; cytoplasm; nuclear chromatin; cytosol; lipid raft

Molecular Function: identical protein binding; signal transducer activity; protein binding; protein phosphatase binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; T-helper 1 cell lineage commitment; negative regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interferon type I production; innate immune response; mammary gland epithelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of isotype switching to IgE isotypes; regulation of cell proliferation