SMCY/JARID1D recombinant protein

Human SMCY/JARID1D

Cat. Num. AAA692086

• Gene Names: KDM5D; HY; HYA; SMCY; JARID1D
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & silver stain
• Synonyms: SMCY/JARID1D; Human SMCY/JARID1D; Recombinant Human SMCY/JARID1D; Lysine-specific demethylase 5D; Histone demethylase JARID1D; Histocompatibility Y antigen; SMCY/JARID1D recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: ALLVALQRLPVRLP
• Sequence Length: 301

• N Terminal Sequence: ALLVALQRLPVRLP
• Buffer: 50 mM NaP, 300 mM NaCl, pH 8.0
• Preparation and Storage: The lyophilized human SMCY, though stable at room temperature, is best stored in working aliquots at -20 degree C to -70 degree C.

Testing Data

Related Product Information for SMCY/JARID1D recombinant protein

H-Y antigen is defined as a male histocompatibility antigen that causes rejection of male skin grafts by female recipients of the same inbred strain of rodents. Male-specific, or H-Y antigen(s), are also detected by cytotoxic T cells and antibodies. H-Y antigen appears to be an integral part of the membrane of most male cells. In addition, H-Y antibodies detect a soluble form of H-Y that is secreted by the testis. The gene (Smcy/SMCY) coding for H-Y antigen detected by T cells has been cloned. It is expressed ubiquitously in male mice and humans, and encodes an epitope that triggers a specific T-cell response in vitro. Additional epitopes coded for by different Y-chromosomal genes are probably required in vivo for the rejection of male grafts by female hosts. The molecular nature of H-Y antigen detected by antibodies on most male cells is not yet known. Testis-secreted, soluble H-Y antigen, however, was found to be identical to Mullerian-inhibiting substance (MIS). MIS cross-reacts with H-Y antibodies and identical findings were obtained for soluble H-Y antigen and MIS, i.e., secretion by testicular Sertoli and, to a lesser degree, ovarian cells, binding to a gonad-specific receptor, induction of gonadal sex reversal in vitro and, in cattle, in vivo. H-Y antisera also detect a molecule or molecules associated with the heterogametic sex in non-mammalian vertebrates. Molecular data on this antigen or antigens are not yet available.

Product Categories/Family for SMCY/JARID1D recombinant protein

Cytokines & Growth Factors

References

1. Wolf U, Cytogenet Cell Genet 80:232, 1998 2. Scott DM et al, J Mol Med (Berl) 75:103, 1997 3. Müller U, Hum Genet 97(6):701, 1996 4. Simpson e et al, Annu Rev Immunol 15:39, 1997

NCBI and Uniprot Product Information

• NCBI GI #: 226442991
• NCBI GeneID: 8284
• NCBI Accession #: NP_001140177.1
• NCBI GenBank Nucleotide #: NM_001146705.1
• UniProt Accession #: Q9BY66; Q92509; Q92809; Q9HCU1; A2RU19; A6H8V7; B7ZLX1
• Molecular Weight: 33,87 kDa
• NCBI Official Full Name: lysine-specific demethylase 5D isoform 1
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 5D
• NCBI Official Symbol: KDM5D
• NCBI Official Synonym Symbols: HY; HYA; SMCY; JARID1D
• NCBI Protein Information: lysine-specific demethylase 5D; H-Y; protein SmcY; Smcy homolog, Y-linked; SMC homolog, Y chromosome; histone demethylase JARID1D; histocompatibility Y antigen; jumonji/ARID domain-containing protein 1D; selected mouse cDNA on Y, human homolog of; Jumonji, AT rich interactive domain 1D (RBP2-like)
• UniProt Protein Name: Lysine-specific demethylase 5D
• UniProt Gene Name: KDM5D
• UniProt Synonym Gene Names: HY; HYA; JARID1D; KIAA0234; SMCY; H-Y
• UniProt Entry Name: KDM5D_HUMAN

NCBI Description

This gene encodes a protein containing zinc finger domains. A short peptide derived from this protein is a minor histocompatibility antigen which can lead to graft rejection of male donor cells in a female recipient. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Uniprot Description

Function: Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May play a role in spermatogenesis. Ref.10 Ref.11 Ref.12

Cofactor: Ascorbate. Ref.10Binds 1 Fe2+ ion per subunit. Ref.10

Subunit structure: Interacts with PCGF6 and MSH5. Ref.10 Ref.13

Subcellular location: Nucleus

Probable.

Domain: The JmjC domain is required for enzymatic activity.

Sequence similarities: Belongs to the JARID1 histone demethylase family.Contains 1 ARID domain.Contains 1 JmjC domain.Contains 1 JmjN domain.Contains 2 PHD-type zinc fingers.

Sequence caution: The sequence BAA13241.2 differs from that shown. Reason: Erroneous initiation.