Spondin 1 (SPON1) Recombinant Protein | SPON1 recombinant protein

Recombinant Spondin 1 (SPON1)

Cat. Num. AAA2010420

• Gene Names: Spon1; FSP; AI666765; AW455831; BC020531; D330035F22Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Spondin 1 (SPON1); Recombinant Spondin 1 (SPON1); SPON1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEFELRRQ-PILDCC ACGTAKYRLT FYGNWSEKTH PKDYPRRANH WSAIIGGSHS KNYVLWEYGG YASEGVKQVA ELGSPVKMEE EIRQQSDEVL TVIKAKAQWP AWQPVNVRAA PSAEFSVDRT RHLMSFLTMM GPSPDWNVGL SAEDLCTKEC GWVQKVVQDL IPWDAGTDSG VTYESPNKPT IPQEKIRPLT SLDHPQSPFY DP
• Sequence Length: 807

• Applicable Applications for SPON1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Pro195~Pro392 (Accession # Q8VCC9) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 21704174
• NCBI GeneID: 233744
• NCBI Accession #: NP_663559.1
• NCBI GenBank Nucleotide #: NM_145584.2
• UniProt Accession #: Q8VCC9; Q8K2Q8
• Molecular Weight: 28.6kDa
• NCBI Official Full Name: spondin-1
• NCBI Official Synonym Full Names: spondin 1, (f-spondin) extracellular matrix protein
• NCBI Official Symbol: Spon1
• NCBI Official Synonym Symbols: FSP; AI666765; AW455831; BC020531; D330035F22Rik
• NCBI Protein Information: spondin-1; F-spondin
• UniProt Protein Name: Spondin-1
• Protein Family: Spondin
• UniProt Gene Name: Spon1
• UniProt Entry Name: SPON1_MOUSE

Uniprot Description

SPON1: Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Biological Process: cell adhesion